脾切除肝硬化门脉高压大鼠血浆TNF-α质量浓度的变化

首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (2): 246-249.

脾切除肝硬化门脉高压大鼠血浆TNF-α质量浓度的变化

张淑文, 刘东斌

首都医科大学宣武医院普外科

收稿日期:2003-09-23 修回日期:1900-01-01 出版日期:2004-04-15 发布日期:2004-04-15

Effect of Tumor Necrosis Factor on Venous Plasma of Portal Hypertension Rat Perserved Splen

Zhang Shuwen, Liu Dongbin

Department of General Surgery, Xuanwu Hospital, Capital University of Medical Sciences

Received:2003-09-23 Revised:1900-01-01 Online:2004-04-15 Published:2004-04-15

摘要/Abstract

摘要： 为研究脾切除术、脾动脉结扎术对肝硬化门脉高压症(PHT)大鼠血浆肿瘤坏死因子(TNF-α)的影响,研究PHT时TNF-α与一氧化氮合酶(NOS)间的关系,建成PHT大鼠模型,用放射免疫分析法动态检测TNF-α,根据吸光度的大小测定血浆NOS活性,并观察术后4周内各组的病死率。发现:PHT大鼠血浆TNF-α质量浓度较正常对照组明显增高(P<0.01);PHT脾切除组术后3d、7d、14d血浆TNF-α质量浓度与实验对照组比较有显著性差异(P<0.01);脾动脉结扎组TNF-α的质量浓度虽比实验对照组升高,但无显著性差异。实验对照组血浆NOS活性明显高于正常对照组(P<0.01),且与TNF-α显著正相关(P<0.01,r=0.946);脾动脉结扎组NOS活性显著低于实验对照组及脾切除组(P<0.01)。PHT时血浆TNF-α的质量浓度显著增高,提示:TNF-α可能在PHT形成中起重要作用。与脾动脉结扎组相比较,脾切除组血浆TNF-α的质量浓度显著增高,血浆NOS活性以及病死率亦显著增高,提示:脾动脉结扎术后脾脏仍可发挥一定作用,PHT时应保留脾脏。

关键词: 肝硬化门脉高压症, 肿瘤坏死因子, 脾切除术, 脾动脉结扎术

Abstract: The aim was to study the effect of portal hypertension (PHT) on tumor necrosis factor (TNF-α) in venous plasma, the influence of splenectomy and splenic artery ligation on TNF-α levels in venous plasma in model rats and to investigate the relation between TNF-α and NOS levels in venous plasma in PHT rats. Healthy Wistar rats were randomized into four groups: The latter three groups were used to established PHT model. TNF-α levels in venous plasma were dynamically measured by radioimmunoassay (RIA) at points after the splenectomy and SAL; NOS levels in venous plasma were determined 14 d after operation. The mortality between SLC group and SAL group were compared 4 weeks after operation. Light microscope were applied to observe the pathological change. PHT TNF-αlevels in venous plasma were significantly higher than NCG group (P<0.01). SLC group TNF-αin plasma increased significantly, compared with MCG group(P<0.01), at points (3 d, 7 d, and 14 d) after splenectomy. SAL group TNF-α levels in venous plasma were slightly higher than MCG group (P> 0.05), and were significantly lower than SLC group (P< 0.01) at points (3 d, 7 d, 14 d) after SAL. MCG group NOS levels in venous plasma were significantly elevated(P<0.01), compared with NCG group, there was a positive correlation(r=0.946, P<0.01) between TNF-α levels and NOS levels in venous plasma, while SAL group NOS levels were significantly reduced (P< 0.01), compared with MCG group and SLC group respectively. The morbidity in SLC group was remarkably higher than that of SAL group (P< 0.05). In PHT, TNF-α levels in venous plasma significantly increase, possibly play an important role in the course of PHT. TNF-αlevels in venous plasma significantly increase after splenectomy, while the morbidity also elevate remarkably. Compared with PHT model rats, SAL group TNF-α levels and the morbidity don't increas significantly, and prognosis can be improved.

Key words: cirrhotic portal hypertension, tumor necrosis factor, splenectomy, splenic artery ligation

中图分类号:

张淑文;刘东斌. 脾切除肝硬化门脉高压大鼠血浆TNF-α质量浓度的变化[J]. 首都医科大学学报, 2004, 25(2): 246-249.

Zhang Shuwen;Liu Dongbin. Effect of Tumor Necrosis Factor on Venous Plasma of Portal Hypertension Rat Perserved Splen[J]. Journal of Capital Medical University, 2004, 25(2): 246-249.

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