肿瘤坏死因子α诱导蛋白2在过敏性鼻炎小鼠模型中的表达及与血管生成的关系

doi:10.3969/j.issn.1006-7795.2013.06.002

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (6): 790-794.doi: 10.3969/j.issn.1006-7795.2013.06.002

• 过敏性鼻炎和慢性鼻窦炎 • 上一篇下一篇

肿瘤坏死因子α诱导蛋白2在过敏性鼻炎小鼠模型中的表达及与血管生成的关系

王敏^1,2, 杨军^1,2, 尚军³, 张罗^1,2

1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科, 北京 100730;
2. 北京市耳鼻咽喉科研究所基础室, 耳鼻咽喉头颈科学教育部重点实验室, 北京 100005;
3. 首都医科大学附属北京同仁医院中心实验室, 北京 100730

收稿日期:2013-10-08 出版日期:2013-12-21 发布日期:2013-12-13
通讯作者: 张罗 E-mail:dr.luozhang@gmail.com
基金资助:
国家杰出青年科学基金（81025007）；首都医科大学附属北京同仁医院科研基金（2012-YJJ-002）。

Expression of tumor necrosis factor-α-inducible protein-2 in a mouse model of allergic rhinitis and its relationship with angiogenesis

WANG Min^1,2, YANG Jun^1,2, SHANG Jun³, ZHANG Luo^1,2

1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730 China;
2. Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Institute of Otorhinolaryngology, Beijing 100005, China;
3. Central Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Received:2013-10-08 Online:2013-12-21 Published:2013-12-13
Supported by:
This study was supported by the National Science Foundation for Distingnished Young Scholars of China(81025007);Foundation of Beijing Tongren Hospital Capital Medical University(2012-YJJ-002).

摘要/Abstract

摘要：

目的观察过敏性鼻炎中肿瘤坏死因子α诱导蛋白2（tumor necrosis factor-α-inducible protein-2，TNFAIP2）的表达及与血管生成的关系。方法建立卵清蛋白（ovalbumin，OVA）诱导的过敏性鼻炎小鼠模型，检测TNFAIP2和血管内皮细胞标记（CD31）的表达，并分析相关性。结果成功建立OVA诱导的过敏性鼻炎小鼠模型，表现为小鼠挠鼻次数、血清OVA特异性IgE浓度、鼻黏膜局部嗜酸细胞和肥大细胞的聚集均显著高于正常对照。TNFAIP2 mRNA和蛋白及CD31mRNA在过敏性鼻炎小鼠鼻黏膜局部的表达均明显高于正常对照。TNFAIP2和CD31mRNA的表达存在正相关。结论 TNFAIP2参与了过敏性鼻炎的发生，且可能与血管生成有关。

关键词: 肿瘤坏死因子α诱导蛋白2, 血管生成, 过敏性鼻炎

Abstract:

Objective To investigate the expression of tumor necrosis factor-α-inducible protein-2(TNFAIP2) in allergic rhinitis and its relationship with angiogenesis. Methods Allergic rhinitis mouse model was induced by ovalbumin(OVA), expression of TNFAIP2 and CD31(the marker of endothelial cells) in nasal mucosa was measured and correlation analysis was done. Results The number of nasal rubbing, serum OVA-specific IgE levels, and nasal mucosal eosinophil and mast cell infiltration were significantly higher in allergic rhinitis mouse model than in the control mice. Compared to the control mice, expression of TNFAIP2 mRNA and protein and CD31 mRNA in allergic rhinitis mouse model increased significantly. TNFAIP2 expression positively correlated with CD31. Conclusion TNFAIP2 may be involved in the development of allergic rhinitis via regulating the angiogenesis.

Key words: tumor necrosis factor-α-inducible protein-2, angiogenesis, allergic rhinitis

中图分类号:

R392.8

王敏, 杨军, 尚军, 张罗. 肿瘤坏死因子α诱导蛋白2在过敏性鼻炎小鼠模型中的表达及与血管生成的关系[J]. 首都医科大学学报, 2013, 34(6): 790-794.

WANG Min, YANG Jun, SHANG Jun, ZHANG Luo. Expression of tumor necrosis factor-α-inducible protein-2 in a mouse model of allergic rhinitis and its relationship with angiogenesis[J]. Journal of Capital Medical University, 2013, 34(6): 790-794.

参考文献

[1] Lim Y S, Won T B, Shim W S, et al. Induction of airway remodeling of nasal mucosa by repetitive allergen challenge in a murine model of allergic rhinitis[J]. Ann Allergy Asthma Immunol, 2007, 98(1):22-31.
[2] Nakaya M, Dohi M, Okunishi K, et al. Prolonged allergen challenge in murine nasal allergic rhinitis: nasal airway remodeling and adaptation of nasal airway responsiveness[J]. Laryngoscope, 2007, 117(5):881-885.
[3] Moon I J, Kim D Y, Rhee C S, et al. Role of angiogenic factors in airway remodeling in an allergic rhinitis murine model[J]. Allergy Asthma Immunol Res, 2012, 4(1):37-45.
[4] Kirmaz C, Ozbilgin K, Yuksel H, et al. Increased expression of angiogenic markers in patients with seasonal allergic rhinitis[J]. Eur Cytokine Netw, 2004, 15(4):317-322.
[5] Sarma V, Wolf F W, Marks R M, et al. Cloning of a novel tumor necrosis factor-alpha-inducible primary response gene that is differentially expressed in development and capillary tube-like formation in vitro[J]. J Immunol, 1992, 148(10):3302-3312.
[6] Ma Y, Koza-Taylor P H, DiMattia D A, et al. Microarray analysis uncovers retinoid targets in human bronchial epithelial cells[J]. Oncogene, 2003, 22(31):4924-4932.
[7] Cooper P, Potter S, Mueck B, et al. Identification of genes induced by inflammatory cytokines in airway epithelium[J]. Am J Physiol Lung Cell Mol Physiol, 2001, 280(5):L841-852.
[8] Chen L C, Chen C C, Liang Y, et al. A novel role for TNFAIP2: its correlation with invasion and metastasis in nasopharyngeal carcinoma[J]. Mod Pathol, 2011, 24(2):175-184.
[9] Liu Z, Wei S, Ma H, et al. A functional variant at the miR-184 binding site in TNFAIP2 and risk of squamous cell carcinoma of the head and neck[J]. Carcinogenesis, 2011, 32(11):1668-1674.
[10] Kondratiev S, Duraisamy S, Unitt C L, et al. Aberrant expression of the dendritic cell marker TNFAIP2 by the malignant cells of Hodgkin lymphoma and primary mediastinal large B-cell lymphoma distinguishes these tumor types from morphologically and phenotypically similar lymphomas[J]. Am J Surg Pathol, 2011, 35(10):1531-1539.
[11] KleinJan A, Willart M, van Nimwegen M, et al. United airways: circulating Th2 effector cells in an allergic rhinitis model are responsible for promoting lower airways inflammation[J]. Clin Exp Allergy, 2010, 40(3):494-504.
[12] Wang M, Zhang W, Shang J, et al. Immunomodulatory effects of IL-23 and IL-17 in a mouse model of allergic rhinitis[J]. Clin Exp Allergy, 2013, 43(8):956-966.
[13] Konno M, Hamazaki T S, Fukuda S, et al. Efficiently differentiating vascular endothelial cells from adipose tissue-derived mesenchymal stem cells in serum-free culture[J]. Biochem Biophys Res Commun, 2010, 400(4):461-465.
[14] Liu Y, Lu X, Yu H J, et al. The expression of osteopontin and its association with Clara cell 10 kDa protein in allergic rhinitis[J]. Clin Exp Allergy, 2010, 40(11):1632-1641.
[15] Baraniuk J N. Pathogenesis of allergic rhinitis[J]. J Allergy Clin Immunol, 1997, 99(2):S763-772.
[16] Matsune S, Ohori J, Sun D, et al. Vascular endothelial growth factor produced in nasal glands of perennial allergic rhinitis[J]. Am J Rhinology, 2008, 22(4):365-370.
[17] Mori S, Fujieda S, Sunaga H, et al. Expression of platelet-derived endothelial cell growth factor and vascularity in the nasal mucosa from allergic rhinitis[J]. Clin Exp Allergy, 2000, 30(11):1637-1644.
[18] Sarma V, Wolf F W, Marks R M, et al. Cloning of a novel tumor necrosis factor-alpha-inducible primary response gene that is differentially expressed in development and capillary tube-like formation in vitro[J]. J Immunol, 1992, 148(10):3302-3312.

肿瘤坏死因子α诱导蛋白2在过敏性鼻炎小鼠模型中的表达及与血管生成的关系

Expression of tumor necrosis factor-α-inducible protein-2 in a mouse model of allergic rhinitis and its relationship with angiogenesis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	程姣姣, 阮祥燕, 杜娟, 谷牧青. 间充质干细胞对人冻融卵巢组织移植后低氧期保护作用的研究进展[J]. 首都医科大学学报, 2020, 41(4): 536-541.
[2]	颜岑, 罗小敏, 冯英梅. 胰高血糖素样肽1通过类血管生成因子4调节糖尿病db小鼠棕色脂肪活性的机制[J]. 首都医科大学学报, 2020, 41(2): 217-224.
[3]	杨龙艳, 王岩, 许月超, 程雅楠, 马燕, 赵冬. 血管生成素样蛋白4在非酒精性脂肪肝诊断中的应用效果分析[J]. 首都医科大学学报, 2020, 41(1): 31-34.
[4]	李景云, 张媛, 张罗. NOS2基因单核苷酸多态性与单纯尘螨过敏性鼻炎相关性研究[J]. 首都医科大学学报, 2018, 39(1): 51-56.
[5]	杨军, 王敏, 张罗. 细胞因子和免疫球蛋白抗体在过敏性鼻炎小鼠早期无症状阶段和晚期有症状阶段表达的差异[J]. 首都医科大学学报, 2018, 39(1): 63-68.
[6]	娄鸿飞, 马思远, 赵岩, 曹飞飞, 贺飞, 王向东, 王成硕, 张罗. 京津冀地区自报过敏性鼻炎病人吸入过敏原检测临床相关性分析[J]. 首都医科大学学报, 2017, 38(5): 665-670.
[7]	马思远, 娄鸿飞, 王成硕, 张罗. 北京地区可疑过敏性鼻炎病人吸入性变应原特征分析[J]. 首都医科大学学报, 2017, 38(5): 671-676.
[8]	赵延明, 王成硕, 赵亚丽, 张媛, 张罗. 人类白细胞抗原Ⅱ类基因多态性与中国人群尘螨过敏性鼻炎相关性研究[J]. 首都医科大学学报, 2017, 38(5): 688-694.
[9]	赵亚丽, 张媛, 王成硕, 张罗. 白细胞介素-6受体基因非同义变异rs2228145和中国过敏性鼻炎病人的相关性研究[J]. 首都医科大学学报, 2017, 38(5): 695-699.
[10]	赵延明, 张罗. 过敏性鼻炎的遗传学研究进展[J]. 首都医科大学学报, 2013, 34(6): 808-813.
[11]	王鹏龙;程亚涛;汪林;徐士勋;王艳慧;李强;雷海民. 齐墩果酸和去氧胆酸衍生物合成及抗血管生成活性研究[J]. 首都医科大学学报, 2012, 33(4): 494-497.
[12]	张媛;刘承耀;段甦;赵延明;张罗. 过敏性鼻炎皮肤点刺试验阳性界值对血清特异性IgE诊断价值的影响[J]. 首都医科大学学报, 2011, 32(6): 717-720.
[13]	段甦;张媛;张罗. 过敏性疾病的自然进程及干预[J]. 首都医科大学学报, 2011, 32(1): 55-59.
[14]	杨酉;刘仲燕;韩德民;张罗. 非过敏性鼻炎伴嗜酸性粒细胞增多综合征患者的鼻通气功能评估[J]. 首都医科大学学报, 2011, 32(1): 26-31.
[15]	张媛;赵延明;贺飞;张罗. 儿童过敏性鼻炎的吸入性过敏原[J]. 首都医科大学学报, 2011, 32(1): 13-16.