使用配置荧光探测器的HPLC法分析血液中的异丙酚

首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (4): 454-456.

使用配置荧光探测器的HPLC法分析血液中的异丙酚

崔秀生¹, 宋晓虹¹, 陈怡¹, 张炳熙², 董钺²

1. 首都医科大学化学生物学药学院;2. 首都医科大学附属北京同仁医院麻醉科

收稿日期:2003-11-30 修回日期:1900-01-01 出版日期:2004-10-15 发布日期:2004-10-15

Quantitation of Propofol in Blood by HPLC with Fluorimeter

Cui Xiusheng¹, Song Xiaohong¹, Chen Yi¹, Zhang Bingxi², Dong Yue²

1. College of Chemical Biology and Pharmacology, Capital University of Medical Sciences;2. Department of Anaesthetic, Beijing Tongren Hospital, Affiliate of Capital University of Medical Sciences

Received:2003-11-30 Revised:1900-01-01 Online:2004-10-15 Published:2004-10-15

摘要/Abstract

摘要： 使用荧光探测HPLC法,测定了临床血样中异丙酚的质量浓度.全血样品用正己烷提取后,再用0.4 mol/L NaOH甲醇溶液反提.使用C18 ODS柱(250 mm×4.6 mm) ;乙腈-水(体积分数分别为60%、40%,每1 000 mL加1 mL三氟乙酸)为流动相;以百里酚作为内标;激发波长276 nm,发射波长310 nm荧光检测.结果:方法的最低检测下限0.5 μg/L,平均相对回收率在93%～104%之间,RSD(相对标准偏差)<9.2%.

关键词: 异丙酚, 血药浓度, HPLC分析法

Abstract: The propofol in human blood was measured by HPLC with fluorimeter. Thymol was used as an internal standard. Following the extraction with hexane, propofol was extracted from the hexane into the NaOH methanol solution(0.4 mol/L). Chromatographic condition: a C18 ODS column(250 mm×4.6 mm, 5 μm) was used; the mobile phase was a mixture solution of acetonitrile and water (60%,40%, added 1 mL of trifluoroacetic acid per 1 000 mL); the excitation and emission wave lengths were 276 and 310 nm respectively. The limit of quantification is 0.5 μg/L, average recovery is between 93% and 104%, RSD <9.2%.

Key words: propofol, concentration in blood, HPLC

中图分类号:

O625.31

崔秀生;宋晓虹;陈怡;张炳熙;董钺. 使用配置荧光探测器的HPLC法分析血液中的异丙酚[J]. 首都医科大学学报, 2004, 25(4): 454-456.

Cui Xiusheng;Song Xiaohong;Chen Yi;Zhang Bingxi;Dong Yue. Quantitation of Propofol in Blood by HPLC with Fluorimeter[J]. Journal of Capital Medical University, 2004, 25(4): 454-456.

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