人脐带血细胞分化为神经样细胞的实验研究

首都医科大学学报 ›› 2005, Vol. 26 ›› Issue (1): 55-58.

人脐带血细胞分化为神经样细胞的实验研究

季凤清¹, 孙海梅¹, 曾晓蓓¹, 李宝红¹, 张华¹, 王秀琴¹, 杨慧², 鲁玲玲², 赵春礼²

1. 首都医科大学组织胚胎学教研室;2. 首都医科大学神经科学研究所

收稿日期:2004-09-03 修回日期:1900-01-01 出版日期:2005-02-24 发布日期:2005-02-24
通讯作者: 季凤清

Expansion and Differentiation of Human Umbilical Cord Blood Cells Into Neuronlike Cells

Ji Fengqing¹, Sun Haimei¹, Zeng Xiaobei¹, Li Baohong¹, Zhang Hua¹, Wang Xiuqin¹, Yang Hui², Lu Lingling², Zhao Chunli²

1. Department of Histology and Embryology, Capital University of Medical Sciences;2. Institute of Neuron Science, Capital University of Medical Sciences

Received:2004-09-03 Revised:1900-01-01 Online:2005-02-24 Published:2005-02-24

摘要/Abstract

摘要：

为探讨人脐带血细胞分化为神经样细胞的可行性, 无菌条件下采集正常人脐血50～100mL置于加有抗凝剂的采血袋中, 分离脐血单个核细胞进行培养, 并加入细胞因子加以诱导分化, 利用免疫细胞化学方法进行鉴定。结果显示:脐血单个核细胞能在体外培养中增生分化和表达干细胞特异性抗原神经巢蛋白(nestin), 并最终分化为神经元样和神经胶质样细胞。免疫组化染色可见神经元特异性烯醇化酶(NSE)和胶质原性纤维酸性蛋白(GFAP)抗原表达。碱性成纤维细胞生长因子(bFGF)和表皮生长因子(EGF)协同作用对脐血细胞的分化有促进作用。提示:脐血单个核细胞具有增生和分化潜能, 可分化为神经元样及神经胶质细胞样细胞, 可以作为神经细胞的种子细胞。

关键词: 脐带血, 诱导分化, 神经细胞

Abstract:

The aim was to explore the isolation, purification and differentiation of umbil ical cord blood cells into neuronlick cells in vitro. Human cord blood samples were obtained, cultured and induced with fectors. Identification was performed with immunochemistry method. Umbilical cord blood could proliferate an d generate clones when cultured in vitro. These cell grew rapidly and different iated into neuronlike cells and astrocytelike cells. The cell clones proliferate d from stem cell could express nestin antigen and the differentiated cells expre ssing GFAP or NSE antigen respectively. In this study, bFGF and EGF in fluencing the induction and differentiation of Umbilical cord blood were observed. It suggests that umbilical cord blood cells have selfrenewal and differentiat ion abilities. They might differentiate into neuronlike and astrocytelike cells which express GFAP or NSE. The umbilical cord blood can be considered the ideal seed cells of the neural stem cells.

Key words: umbilical cord blood, induction diff erentiation, neuronlike cell

中图分类号:

R329.2⁺4

季凤清;孙海梅;曾晓蓓;李宝红;张华;王秀琴;杨慧;鲁玲玲;赵春礼. 人脐带血细胞分化为神经样细胞的实验研究[J]. 首都医科大学学报, 2005, 26(1): 55-58.

Ji Fengqing;Sun Haimei;Zeng Xiaobei;Li Baohong;Zhang Hua;Wang Xiuqin;Yang Hui;Lu Lingling;Zhao Chunli. Expansion and Differentiation of Human Umbilical Cord Blood Cells Into Neuronlike Cells[J]. Journal of Capital Medical University, 2005, 26(1): 55-58.

[1]	陈思, 周催, 余辉艳, 陶领伟, 王玉珊, 肖荣. 27-羟基胆固醇对神经细胞和星形胶质细胞溶酶体胆固醇转运相关因子表达的影响[J]. 首都医科大学学报, 2019, 40(2): 269-275.
[2]	文勇;Yongil KWON. 乙醇通过激活JNK和p38K引起成神经瘤细胞死亡[J]. 首都医科大学学报, 2012, 33(6): 814-817.
[3]	廖秋菊;王晶;秦俭. 胰岛素防治大鼠一氧化碳中毒迟发性脑病的实验研究[J]. 首都医科大学学报, 2012, 33(2): 233-237.
[4]	万伟庆;任同;王夏;裴傲;张鹏飞;马振宇;张亚卓;王忠诚. 中枢神经细胞瘤和少突胶质细胞瘤基因差异表达的研究[J]. 首都医科大学学报, 2008, 29(2): 190-193.
[5]	杨健;贾军;汪璇;王晓民. 体外诱导成体大鼠骨骼肌组织来源的干细胞向神经干细胞的转化[J]. 首都医科大学学报, 2007, 28(3): 318-323.
[6]	殷娟娟;韩俊燕;李昕;李尧华;刘耀波;于顺;. α-突触核蛋白功能片段向多巴胺能神经细胞内的转运及其对细胞增殖的影响[J]. 首都医科大学学报, 2007, 28(3): 345-349.
[7]	张晨;李昕;李尧华;韩俊燕;陈彪;于顺;. 细胞外α-突触核蛋白对MES23.5多巴胺能神经细胞的促增殖作用[J]. 首都医科大学学报, 2006, 27(5): 616-619.
[8]	张杰;吴萍;李洁;宣登峰;李国君. 硒对不同价态锰诱导的SH-SY5Y神经细胞脂质过氧化的影响[J]. 首都医科大学学报, 2004, 25(4): 421-423.
[9]	季凤清;孙海梅;岳旭;郭艳茹;杜晓宇;郭崇洁;赵天德. 一氧化氮在缺氧复氧所致神经细胞凋亡中的作用及银杏叶提取物的保护效应[J]. 首都医科大学学报, 2001, 22(3): 193-198.