关键词: 血小板, 膜糖蛋白受体, 基因多态性, 缺血性脑血管病

Abstract: Objective To research the association between the polymorphisms of platelet glycoprotein receptorⅠbα and ischemic cerebrovascular disease.Methods A hospital based case-control study was conducted of 232 cases in cerebrovascular diseases group confirmed by brain CT or MRI and 228 cases without clinical evidence of CVD in control group. By Polymerase chain reaction(PCR) amplification of genomic DNA, to evaluate the interaction between the GPⅠbα genotype and other variables in relation to the prevalence of CVD.Results 591 bp PCR product of HPA-2 was digested with restriction enzyme which recognizes homozygous MM,TT and heterozygous T/M. Genotypes with 145M(T/M,MM) were 20.7% in CVD group, compared with that of the control group(7.0%). Statistics signs showed significant differences( χ²=17.946, P =0.000 2). Genotypes with 145M(T/M,MM) were 9.8% in cerebral embolism group, 17.5% in lacunar infarction group, 38.2% in large blood vessel occlusion group. When separate analysis were performed, statistics signs showed no significant differences between cerebral embolism group and control group( χ²=0.465, P =0.495). Contrast, statistics signs showed significant differences between lacunar infarction group, large blood vessel occlusion group and control group( χ²=9.236, P =0.002; χ²=37.87, P = 0.000 1 ). We put the glycoproteinⅠbαHPA-2 as the dependent variable, other cerebrovascular disease risks as independent variable. By logistic regression analysis, to remove an influence of these factors.Conclusion The glycoproteinⅠbα polymorphisms is one of the independent risks of ischemic cerebrovascular diseases.

Key words: platelet, membrane glycoprotein, polymorphisms of gene, ischemic cerebrovascular disease