40例泌尿生殖道念珠菌病患者的菌种及药敏分析

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (2): 266-269.

40例泌尿生殖道念珠菌病患者的菌种及药敏分析

崔羽立, 赵俊英

首都医科大学附属北京友谊医院皮肤性病科

收稿日期:2005-09-07 修回日期:1900-01-01 出版日期:2006-04-24 发布日期:2006-04-24

Analyses on Isolation and Identification and Drug Sensitivity of the Clinical Strains from Patients with Uro-genital Candidiasis

Cui Yuli, Zhao Junying

Department of Dermatology, Beijing Friendship Hospital, Capital University of Medical Sciences

Received:2005-09-07 Revised:1900-01-01 Online:2006-04-24 Published:2006-04-24

摘要/Abstract

摘要： 目的了解泌尿生殖道念珠菌病的菌群分布及体外抗真菌药敏情况.验证科玛嘉念珠菌显色培养基临床应用的可行性.方法 40例菌株均来自北京友谊医院皮肤科和妇产科门诊,临床症状典型,真菌镜检阳性.应用科玛嘉念珠菌显色培养基进行菌种鉴定.利用微生物自动鉴定系统VITEK.YBC酵母菌鉴定卡,进行菌种鉴定.用酵母样真菌最低抑菌浓度(MIC)药敏试剂盒进行MIC值测定.结果对培养阳性的40例菌株,用科玛嘉念珠菌显色培养基菌种鉴定白念珠菌37例,非白念珠菌3例,用微生物自动鉴定系统YBC酵母菌鉴定卡菌种鉴定白念珠菌37例,近平滑念珠菌2例,隐球菌1例.对从临床分离的39株念珠菌进行7种抗真菌药物MIC敏感性测定,药敏检测结果二性霉素B为71.79%,酮康唑为100%,咪康唑为84.62%,伊曲康唑为41.03%,氟康唑为97.44%,5-氟胞嘧啶为97.44%,特比奈芬为84.62%.结论 1)在生殖道念珠菌病的病原体中,白念珠菌仍为第一位致病菌,占念珠菌的94.87%(37/39).2)科玛嘉念珠菌显色培养基和微生物自动鉴定系统YBC酵母菌鉴定卡在白念珠菌的鉴定方面吻合率可达到100%.

关键词: 生殖道念珠菌病, 抗真菌药敏试验, 菌群分布

Abstract: Objective To investigate the distribution and the drug-sensitivity of Candida SPP in the patients with uro-genital candidiasis in vitro in our hospital,and assess the clinical feasibility of to Candida Chromogenic & lsolation Medium.Methods 40 clinical strains from patients with uro-genital candidiasis in the dermatology department and the gynecology department in our hospital.Candida Chromogenic & lsolation Medium and microorganism automatic testing system were used to identify Candida species,and according to the NCCLS M-27A scheme with typical clinical symptoms,40 strains were studied,including 39 isolates from patients,1 quality control strain.Results 1) 37 of 40 isolates were candida albicans,2 of isolates were candida parapsilosis,and 1 of isolates was Cryptococcus laurentii.2) Drug sensitivity rate of yeast to amphotericin B,ketoconazole,miconazole,itraconazole,fluconazole,cytosine,terbinafine were 71.79%,100%,84.62%,41.03%,97.44%,97.44%,84.62% respectively.Conclusion 1) Among the clinical strains,Candida albicans was the most important pathomycete,and it had a high frequency in uro-genital candidiasis.2) indetification rate of Candida albicans was 100% with Candida Chromogenic & lsolation Medium coincides with microorganism automatic testing system.

Key words: genital candidosis, antifungal drug susceptibility microbial sensitivity tests, distribution of Candida SPP

中图分类号:

R756.5

崔羽立;赵俊英. 40例泌尿生殖道念珠菌病患者的菌种及药敏分析[J]. 首都医科大学学报, 2006, 27(2): 266-269.

Cui Yuli;Zhao Junying. Analyses on Isolation and Identification and Drug Sensitivity of the Clinical Strains from Patients with Uro-genital Candidiasis[J]. Journal of Capital Medical University, 2006, 27(2): 266-269.