首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (3): 290-293.

• 专题报道 • 上一篇    下一篇

纤维连接蛋白基因EDA片段在腺样囊性癌细胞中的表达

张建鹏, 李翠英   

  1. 首都医科大学附属北京口腔医院
  • 收稿日期:2006-04-20 修回日期:1900-01-01 出版日期:2006-06-24 发布日期:2006-06-24
  • 通讯作者: 李翠英

The Expression of EDA Region of Fibronectin in Salivary Adenoid Cystic Carcinoma Cells

Zhang Jianpeng, Li Cuiying   

  1. Beijing Stomatology Hospital, Capital University of Medical Sciences
  • Received:2006-04-20 Revised:1900-01-01 Online:2006-06-24 Published:2006-06-24

摘要: 目的 通过纤维连接蛋白(FN)基因EDA片段在不同转移潜能腺样囊性癌(SACC)细胞中的表达,研究EDA片段与SACC细胞生物学特性的关系.方法应用免疫组织化学及半定量反转录聚合酶链反应(RT-PCR)法,分别从蛋白水平及mRNA水平对高、低转移SACC细胞株中FN基因EDA片断进行检测.结果高转移SACC细胞株(SACC-LM)中FN基因EDA片段蛋白及mRNA的表达高于低转移细胞株(SACC-83).结论 FN基因EDA片段的表达程度与SACC细胞不同转移潜能密切相关.

关键词: 腺样囊性癌, 纤维连接蛋白, EDA片段, 转移

Abstract: Objective To investigate the expression of EDA region of fibronectin(FN) in salivary adenoid cystic carcinoma(SACC) cells and deduce the relationship between EDA and SACC biological characteristic.Methods Immunohistochemistry and reverse transcriptase polymerase chain reaction(RT-PCR) techniques were used to detect EDA region proteins and mRNAs in different metastatic potential SACC cells.Results The expression of EDA region proteins and mRNAs in high metastatic SACC-LM cells were higher than those in low metastatic SACC-83 cells.Conclusion The expression of EDA region of FN may play an important role in the invasion and metastasis of SACC.

Key words: salivary adenoid cystic carcinoma(SACC), fibronectin(FN), EDA region, metastasis

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