依托咪酯和咪达唑仑对小鼠海马脑片细胞外信号调节激酶ERK1/2磷酸化水平的影响

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (5): 565-568.

依托咪酯和咪达唑仑对小鼠海马脑片细胞外信号调节激酶ERK1/2磷酸化水平的影响

高海鹰, 张炳熙

首都医科大学附属北京同仁医院麻醉科

收稿日期:2006-07-10 修回日期:1900-01-01 出版日期:2006-10-24 发布日期:2006-10-24
通讯作者: 张炳熙

Effects of Etomidate and Midazolam on Extracellular Signal-regulated Kinase1/2 Phosphorylation in Mice Hippocampal Slices

Gao Haiying, Zhang Bingxi

Deparment of Anesthesiology, Beijing Tongren Hospital, Capital University of Medical Sciences

Received:2006-07-10 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24

摘要/Abstract

摘要： 目的观察依托咪酯和咪达唑仑对小鼠海马脑片细胞外信号调节激酶ERK1/2磷酸化水平的影响.方法 BALB/ C小鼠,雌雄不拘,取其海马制成厚450 μm脑片,分别应用10^-9～10^-4mol/L依托咪酯和咪达唑仑在36 ℃恒温条件下孵育1 h.应用SDS-PAGE和Western blot等技术,用Gel Doc 凝胶成像系统,半定量检测小鼠海马内p-ERK1/2的磷酸化水平.结果与对照组比较,在本研究应用的浓度范围内,咪达唑仑浓度的增加对ERK1/2磷酸化抑制程度无明显影响;在本研究所应用的浓度范围内,依托咪酯浓度的增加对ERK1磷酸化抑制程度无明显影响.随着依托咪酯浓度的增加,对ERK2磷酸化的抑制程度逐渐增加.结论依托咪酯和咪达唑仑可抑制小鼠海马脑片细胞外信号调节激酶ERK1/2磷酸化水平.在本研究应用的浓度范围内,随浓度的增加,依托咪酯对ERK2磷酸化水平的抑制程度逐渐增加.

关键词: 咪达唑仑, 依托咪酯, ERK MAP激酶, 海马

Abstract: Objective To investigate the effects of etomidate and midazolam on extracellular signal-regulated kinase1/2 phosphorylation in mice hippocampus slices.Methods BALB/C mice weighting 18～22 g were decapitated and the hippocampus were dissected.Hippocampal slices were prepared with MacⅡwain tissue chopper.Hippocampal slices were incubated at 36 ℃ during the whole experiment.The slices were incubated with 10^-9～10^-4mol/L etomidate and midazolam respectively for 1h during the experiment.Phosphorylation of ERK1/2 was examined in hippocampus slices by immunoblotting with both phosphor-p44/42 Map kinase and p44/42 Map kinase antibodies.Experiments were performed in the absence(control) or presence of various concentrations of etomidate and midazolam.Results Clinically relevant concentrations of etomidate and midazolam decrease phosphorylation of ERK1/2.The decrease was concentration-dependent.Conclusion Etomidate and midazolam markedly decrease phosphorylation of ERK1/2 in mice hippocampal slices.

Key words: midazolam, etomidate, ERK MAP kinases, hippocampus

中图分类号:

R614

高海鹰;张炳熙. 依托咪酯和咪达唑仑对小鼠海马脑片细胞外信号调节激酶ERK1/2磷酸化水平的影响[J]. 首都医科大学学报, 2006, 27(5): 565-568.

Gao Haiying;Zhang Bingxi. Effects of Etomidate and Midazolam on Extracellular Signal-regulated Kinase1/2 Phosphorylation in Mice Hippocampal Slices[J]. Journal of Capital Medical University, 2006, 27(5): 565-568.

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