关键词: 骨髓基质细胞, 内皮细胞, 移植, 局灶脑缺血, 动物行为学

Abstract: Objective To investigate therapeutic benefits with intravenous administration of rat bone marrow derived endothelial cells after stroke and assess the improvement of neurologic function.Methods The endothelial cells derived from bone marrow stromal cells(BMSC) of rats,which were multiplied and differentiated in the medium with high concentration of rhgm-CSF(400 μg/L) in vitra.Rats were subjected to permanent cerebral middle artery occlusion(MCAO models,n=45).Intravenous injection via tongue vein with(3×)10⁶ endothelial cells 24 h after stroke for test group(n=15);same amount PBS injection for control group 1(n=15);for control group 2 no intervention after stroke(n=15).Neurologic functional behaviour tests including postural reflex test,limb use asymmetrical test and corner test were performed before transplantation and 1,3,5,7,14 d after stroke. Results Significant recovery of neurological function was found in rats treated with ivadministration of endothelial cells at 7 d and 14 d after stroke,compared with control group 1 and group 2,P<0.05.Conclusion Endothelial cells derived from rats bone marrow could improve neurological function in rats by intravenous injection,the effect starts to be significant on the 7 th day after transplantation,and it is even more significant on the 14 th day,P<0.05,the relevant mechanism should be investigated further in other study.

Key words: bone marrow stromal cell, endothelial cell, transplantation, focal cerebral ischemia, animal behavioural science