关键词: 阿尔茨海默病, 尿激酶型纤溶酶原激活因子, 基因多态性, 聚合酶链反应, 限制性片段长度多态性

Abstract: Objective To explore the relation between the P141L polymorphism of urokinase plasminogen activator gene(PLAU) and Late-onset Alzheimer's disease(LOAD) in Hans.Methods A total of 67 LOAD patients based on the diagnosis standard of DSM-Ⅳ and NINCDS-ADRDA and 71 subjects of the same age and the same sex construction as those in the case group were enrolled in this case-control study.The polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technique was used in detecting the polymorphism of the PLAU.Results The genotype frequencies distribution of both LOAD group and control group was in line with the Hardy-Weinberg law;No significant difference was found between the case group and the control group in terms of the genotype distribution(χ²= 0.223 8,P=0.636 1).No significant difference was found between the two groups in the distribution of allele(χ²= 0.223 8,P=0.636 1).Conclusion No relation was found between polymorphisms of PLAU and the LOAD disease in Hans.

Key words: Alzheimer disease, urokinase plasminogen activator, ! gene polymorphism, polymerase chain reaction, restriction fragment length