关键词: 模型动物, 宫颈癌, 裸鼠, 人乳头状瘤病毒

Abstract: Objective To establish the human cervical cancer animal model through abdominal subcutaneous transplantation in BALB/c nude mice and investigate the rate of tumor-forming and the biological features of the tumor.Methods Human cervical cancer tissue derived from surgical specimens were transplanted into the subcutis of nude mice.The rate of tumor transplantation,generic characteristic,tumor growth of bearing cancer mice were observed every day.On day 85 the tumor-bearing mice were killed and tumor masses were collected for analysis of histological features.Genomic DNAof tumor tissue,peripheral blood,liver,spleen,kidney,omentum,and uterus annex of tumor-bearing BALB/c nude mice were extracted,and then using all organs' genomic DNAas templates,we detected the HPV DNAexpression with polymerase chain reaction(PCR).Results In this experiment,the delitescence period of tumor-forming were 30～35 days.On day 42 the tumor tubercles were visible and grew fast day by day.The average tumor-take rate was 90.5%,and the mean tumor surface areas was(50.0±5.1)mm² on day 80.The transplanted tumors grew well by local invasion.Metastasis was not found.The tumors were analyzed by HEstaining,and the histological appearance of the tumors before and after transplantation were very similar.The positive rate of HPV DNAin the tumor tissue was 100%.The appearance of HPV DNAbefore and after transplantation was the same.The PCRresults definitely displayed the subtype of high-risk human papillomavirus,which suggested cervical cancer patient's HPV16 infection,not HPV18 infection.But HPV DNAwas not detectable in peripheral blood,the spleen,the liver,the kidney,the omentum,and the uterus annex of tumor-bearing BALB/c nude mice.Conclusion Human cervical cancer model in nude mice has been successfully established which simulates the biological features of human cervical cancer.The animal model of human cervical cancer in BALB/c nude mice exhibit a high rate of tumor transplantation,and which may provide a convenient and ideal tool for the research of the mechanism and therapy of human cervical cancer.

Key words: animal model, cervical cancer, nude mice, human papillomavirus