桃金娘油对人鼻腔黏液纤毛传输系统的影响

首都医科大学学报 ›› 2009, Vol. 30 ›› Issue (1): 62-65.

桃金娘油对人鼻腔黏液纤毛传输系统的影响

王楠¹, 韩德民^1,2, 宋晓红¹, 张伟², 张罗^1,2

1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科;2. 北京市耳鼻咽喉科研究所,教育部耳鼻咽喉科学重点实验室

收稿日期:2008-11-18 修回日期:1900-01-01 出版日期:2009-02-21 发布日期:2009-02-21
通讯作者: 张罗

Effects of Myrtol Standardized on Human Nasal Mucociliary Transport

Wang Nan¹, Han Demin^1,2, Song Xiaohong¹, Zhang Wei², Zhang Luo^1,2

1. Department of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University;2. Beijing Institute of Otorhinolaryngology, Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education

Received:2008-11-18 Revised:1900-01-01 Online:2009-02-21 Published:2009-02-21

摘要/Abstract

摘要： 目的通过观察不同浓度桃金娘油对体外培养人鼻腔纤毛运动的作用,以及慢性单纯性鼻炎患者口服标准桃金娘油前后黏液纤毛传输时间的变化,探讨桃金娘油对人鼻腔黏液纤毛传输系统的影响。方法采用组织块法体外培养人鼻腔纤毛细胞,使用高速数字化显微成像技术,观察75 μg/L、150 μg/L和300 μg/L的桃金娘油对纤毛摆动频率的影响。12名慢性鼻炎患者口服标准桃金娘油10 d(300 mg/次,3次/d),用药前后分别测定糖精清除时间,并对其中9名受试者用药前后分别进行相关鼻功能检查。结果 175 μg/L、150 μg/L和300 μg/L的桃金娘油对体外培养的人鼻腔纤毛摆动频率未见显著影响。212名慢性单纯性鼻炎患者口服药物后,糖精清除时间从用药前的(12.8±4.5)min缩短为(9.3±3.8)min(P<0.05)。39名慢性鼻炎患者用药前后鼻阻力和鼻声反射结果差异均有统计学意义(P<0.05)。结论口服标准桃金娘油可改善鼻腔黏液纤毛传输系统功能和鼻腔通气功能,在体外未见75~300 μg/L的桃金娘油对人鼻腔纤毛运动产生明显影响。

关键词: 鼻黏膜, 鼻腔黏液纤毛传输系统, 纤毛, 黏液纤毛传输时间

Abstract: Objective To investigate the effects of Myrtol Standardized on the regulation of healthy human nasal ciliary beat frequency(CBF) and its influence on nasal mucociliary transport time(MTT). Methods Changes in human nasal CBF in response to increasing concentrations of Myrtol Standardized within 20 minutes were quantified by using of high-speed digital microscopy. The MTTs before and after application of Myrtol Standardized in 12 patients with chronic rhinitis were determined by using the saccharin test. 9 patients were examined nasal function test. Results No statistically significant difference was identified when compared to basal CBF at the concentration of 75 μg/L, 150 μg/L or 300 μg/L, Myrtol Standardized. In addition, the use of Myrtol Standardized decreased the mean human nasal MTT from(12.8±4.5) to (9.3±3.8)min (n=12). Conclusion Myrtol Standardized significantly increased MTT in vivo, but no obvious effect on human nasal CBF in vitro was observed.

Key words: nasal mucosa, nasal mucociliary transport, cilia, mucociliary transport time

中图分类号:

R 765.25

王楠;韩德民;宋晓红;张伟;张罗;. 桃金娘油对人鼻腔黏液纤毛传输系统的影响[J]. 首都医科大学学报, 2009, 30(1): 62-65.

Wang Nan;Han Demin;Song Xiaohong;Zhang Wei;Zhang Luo;. Effects of Myrtol Standardized on Human Nasal Mucociliary Transport[J]. Journal of Capital Medical University, 2009, 30(1): 62-65.

[1]	王鸿;矫健;金善哲;张罗. 小鼠鼻腔及气管黏膜上皮细胞的构成和纤毛反应性比较研究[J]. 首都医科大学学报, 2011, 32(1): 21-25.
[2]	矫健;王鸿;金善哲;张罗. 一氧化氮信号通路对小鼠鼻腔纤毛运动的调控[J]. 首都医科大学学报, 2011, 32(1): 17-20.
[3]	王鸿;张伟;矫健;金善哲;张罗. 不同基质对兔气管纤毛上皮细胞贴壁生长的影响[J]. 首都医科大学学报, 2010, 31(5): 604-609.
[4]	矫健;王鸿;孟娜;张罗. L-精氨酸对兔气管上皮细胞纤毛运动的影响[J]. 首都医科大学学报, 2009, 30(6): 729-732.
[5]	刘晨;韩德民;张罗;. 兔气管纤毛蛋白磷酸化的初步研究[J]. 首都医科大学学报, 2009, 30(1): 54-56.
[6]	史凌改;韩德民;张罗;. ATP对人鼻腔纤毛摆动频率的影响[J]. 首都医科大学学报, 2009, 30(1): 57-61.
[7]	矫健;张罗;. 纤毛研究进展[J]. 首都医科大学学报, 2009, 30(1): 70-75.
[8]	杨军;邓秋;周芸;诸小侬. 实验性变应性鼻炎中鼻黏膜组胺的测定[J]. 首都医科大学学报, 2006, 27(1): 109-112.