首都医科大学学报 ›› 2009, Vol. 30 ›› Issue (6): 729-732.doi: 10.3969/j.issn.1006-7795.2009.06.001

• 耳鼻咽喉头颈外科进展 • 上一篇    下一篇

L-精氨酸对兔气管上皮细胞纤毛运动的影响

矫健1, 王鸿1, 孟娜1, 张罗1,2   

  1. 1. 耳鼻咽喉头颈科学教育部重点实验室 北京市耳鼻咽喉科研究所;2. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科
  • 收稿日期:2009-09-22 修回日期:1900-01-01 出版日期:2009-12-21 发布日期:2009-12-21
  • 通讯作者: 张罗

Effects of L-arginine on Ciliary Motility in Rabbit Tracheal Epithelial Cells

JIAO Jian1, WANG Hong1, MENG Na1, ZHANG Luo1,2   

  1. 1. Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education of China, Beijing Institute of Otorhinolaryngology;2. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University
  • Received:2009-09-22 Revised:1900-01-01 Online:2009-12-21 Published:2009-12-21

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322

被引次数

1

摘要：

目的 观察一氧化氮(nitric oxide,NO)前体L-精氨酸(L-arginine,L-arg)对兔气管上皮细胞纤毛摆动的影响。方法 体外培养兔气管上皮组织块,采用高速数字化显微成像技术对纤毛摆动进行定量分析,观察10 mmol/L L-arg及100 μmol/L一氧化氮合酶(nitric oxide synthase,NOS)抑制剂N-硝基-L-精氨酸甲酯(N-nitro-L-arginine methyl ester,L-NAME)对上皮纤毛摆动的影响。结果 1 Hanks平衡盐溶液(Hank's balanced salt solution,HBSS)对照组兔气管上皮细胞纤毛摆动在25 min内无明显变化;2 10 mmol/L L-arg处理组纤毛摆动频率(ciliary beating frequency,CBF)随时间延长逐渐增加,其中加药20 min及25 min后,CBF与加药前相比差异有统计学意义;3 100 μmol/L L-NAME预孵育10 min后再加入10 mmol/L L-arg共同孵育25 min,CBF在各个时间点与对照组相比无明显变化;4 100 μmol/L L-NAME单独作用35 min内CBF无明显变化。结论 L-arg可促进兔气管上皮细胞纤毛摆动,其作用机制是通过激活NOS产生NO而实现的。

关键词: 一氧化氮, 一氧化氮合酶, 精氨酸, 纤毛

Abstract:

Objective To investigate the effects of L-arginine on ciliary beat frequency(CBF) in rabbit tracheal epithelial cells. Methods Cultured rabbit tracheal explants were treated with 10 mmol/L L-arginine and 100 μmol/L N-nitro-L-arginine methyl ester(L-NAME), the CBF was measured by high-speed digital microscopy. Results 1 CBF of epithelial cells in Hank's balanced salt solution(HBSS) control group showed no significant changes in 25 min. 2 CBF increased with time in 10 mmol/L L-arginine-treated group, significant changes were observed at 20 min and 25 min treatment. 3 Pretreatment with 100 μmol/L L-NAME for 10 min blocked the increase of CBF by 10 mmol/L L-arginine, CBF showed no significant changes compared with control group. 4 100 μmol/L L-NAME treatment for 35 min did not change CBF of ciliary cells. Conclusion L-arginine increased CBF of rabbit tracheal epithelial cells via the nitric oxide synthase-nitric oxide(NOS-NO) signal pathway.

Key words: nitric oxide, nitric oxide synthase, arginine, cilia

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