参乌胶囊及其有效成分二苯乙烯苷对老年大鼠海马神经元突触体素表达的影响

首都医科大学学报 ›› 2010, Vol. 31 ›› Issue (1): 60-64.

• 老年病的基础与临床研究 • 上一篇下一篇

参乌胶囊及其有效成分二苯乙烯苷对老年大鼠海马神经元突触体素表达的影响

王蓉^1,2, 赵志炜², 张丽¹, 张兰¹, 李林¹

1. 首都医科大学宣武医院药物研究室, 神经变性病教育部重点实验室;2. 首都医科大学宣武医院中心实验室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-21 发布日期:2010-02-21
通讯作者: 李林

Effects of Shenwu Capsule and Its Effective Component Tetrahydroxystilbene Glucoside on Synaptophysin Expression in Hippocampal Neurons of Aged Rats

WANG Rong^1,2, ZHAO Zhi-wei², ZHANG Li¹, ZHANG Lan¹, LI Lin¹

1. Department of Pharmacology, Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education;2. Central Laboratory, Xuanwu Hospital, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2010-02-21 Published:2010-02-21
Contact: LI Lin

摘要/Abstract

摘要： 目的观察由首都医科大学宣武医院药物研究室自行研制的抗老年性痴呆中药新药参乌胶囊（Shen-wu capsule，SW）及其有效成分何首乌二苯乙烯苷（tetrahydroxystilbene glucoside，TSG）对于老年大鼠海马神经元突触结构和突触体素表达的影响，阐明其防治神经元退行性变的机制。方法选用老年SD大鼠，从21月龄始，分别灌胃给予SW（0.8 g/kg、1.6 g/kg）和TSG（0.03 g/kg、0.06 g/kg）至24月龄。以6月龄大鼠为青年对照，未给药24月龄大鼠为老年对照。应用通道式水迷宫试验检测大鼠学习记忆能力，用电子显微镜观察脑海马神经元突触超微结构，用免疫组织化学染色检测海马神经元突触体素(synaptophysin，SYP)的表达。结果与6月龄青年对照组大鼠相比，24月龄老年大鼠通道式水迷宫试验错误次数明显增多（P<0.01）；给予SW和TSG的老年大鼠在高剂量组可见水迷宫错误反应次数显著减少，与老年对照组相比差异具有统计学意义(P<0.01)。电子显微镜结果显示，24月龄老年大鼠神经元突触数量明显减少；而给予TSG大剂量灌胃组突触数量增多，并可见类似于突触球的结构。免疫组织化学染色结果可见24月龄大鼠SYP染色明显变浅，累积光密度值低(P<0.01)；而灌胃给予SW和大剂量TSG组大鼠SYP表达量明显升高，差异具有统计学意义(P<0.01)。结论参乌胶囊及其有效成分二苯乙烯苷对于老年大鼠的神经元突触靶位具有较好的疗效，本结果为其在临床用于延缓衰老、改善认知功能提供了实验基础。

关键词: 参乌胶囊, 何首乌二苯乙烯苷, 老年, 突触体素

Abstract: Objective To observe the effects of Shen-wu capsule(SW) and its effective component tetrahydroxystilbene glucoside(TSG) on synaptophysin expression in hippocampal neurons of aged rats, and to elucidate the mechanisms of their effects in prevention and treatment in neurodegenerative diseases. Methods SW(0.8 g/kg, 1.6 g/kg) and TSG(0.03 g/kg, 0.06 g/kg) were intragastrically administered to aged Sprague-Dawley rats which were 21 to 24 months old for 3 months. Six-month-old rats were used as young control group, and vehicle-treated 24-month-old rats as aged control group. The straight-channel water maze test was used to evaluate the learning and memory ability in rats. Electron microscopy was used to observe neuronal ultrastructure in hippocampus CA1 area especially the synaptic structure. The expression of synaptophysin(SYP) in hippocampus was detected by immunohistochemical staining. Results Compared with 6-month-old young control group, the number of errors in the water maze test increased in aged control rats at the age of 24 months(P<0.01); and administration of high dose of SW and TSG significantly reduced the number of errors in aged rats(P<0.01). Electron microscopy displayed that the number of synapses in the hippocampus CA1 area clearly diminished in aged control rats, while high dose of TSG administration significantly increased the number of synapses, even formed “synapticglobes”. Immunohistochemical staining showed that the expression of SYP in the hippocampus decreased markedly in aged control rats(P<0.01), but significantly elevated in aged rats treated with SW(low and high doses) and TSG(high dose)(P<0.01). Conclusion SW and TSG protected neuronal synaptic structures and enhanced synaptic protein expression in aged rats, which provided the experimental data for elucidating the anti-aging mechanisms of SW capsule and TSG.

Key words: Shen-wu capsule, tetrahydroxystilbene glucoside, aging, synaptophysin

中图分类号:

R 332， R 338.1⁺3

王蓉;赵志炜;张丽;张兰;李林. 参乌胶囊及其有效成分二苯乙烯苷对老年大鼠海马神经元突触体素表达的影响[J]. 首都医科大学学报, 2010, 31(1): 60-64.

WANG Rong;ZHAO Zhi-wei;ZHANG Li;ZHANG Lan;LI Lin. Effects of Shenwu Capsule and Its Effective Component Tetrahydroxystilbene Glucoside on Synaptophysin Expression in Hippocampal Neurons of Aged Rats[J]. Journal of Capital Medical University, 2010, 31(1): 60-64.

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参乌胶囊及其有效成分二苯乙烯苷对老年大鼠海马神经元突触体素表达的影响

Effects of Shenwu Capsule and Its Effective Component Tetrahydroxystilbene Glucoside on Synaptophysin Expression in Hippocampal Neurons of Aged Rats

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