二黄胶囊对实验性变态反应性脑脊髓炎模型大鼠急性期炎性反应和髓鞘修复的影响

首都医科大学学报 ›› 2011, Vol. 32 ›› Issue (1): 110-115.

二黄胶囊对实验性变态反应性脑脊髓炎模型大鼠急性期炎性反应和髓鞘修复的影响

李康宁¹，樊永平^2*，陈克龙²，周建平³，邵燕³，刘洪艳³，杨文静³

1. 北京中医药大学东直门医院； 2. 首都医科大学附属北京天坛医院中医科； 3. 北京市药物分析研究所药物安全评价中心

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-02-21 发布日期:2011-02-21
通讯作者: 樊永平

Effect of Erhuang Capsule on the Acute Inflammatory Reaction and Myelin Sheath Repairing in Model Rats of Experimental Allergic Encephalomyelitis

LI Kang-ning¹，FAN Yong-ping^2*, CHEN Ke-long², ZHOU Jian-ping³, SHAO Yan³, LIU Hong-yan³, YANG Wen-jing³

1. Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine; 2. Department of Traditional Chinese Medicine, Beijing Tiantan Hospital, Capital Medical University; 3. Center for Drug Safety Evaluation, Beijing Institute for Drug Analysis

Received:1900-01-01 Revised:1900-01-01 Online:2011-02-21 Published:2011-02-21
Contact: FAN Yong-ping

摘要/Abstract

摘要：

目的观察二黄胶囊对实验性变态反应性脑脊髓炎（experimental allergic encephalomyelitis,EAE）模型大鼠的保护和治疗作用。
方法通过用豚鼠脊髓匀浆和完全福氏佐剂1∶1混合，制成乳剂作为免疫抗原，给Lewis大鼠4足垫内注射，建立大鼠EAE模型。观察二黄胶囊对EAE大鼠发病情况、神经组织病理变化及其血清髓鞘碱性蛋白（myelin basic protein,MBP）水平的影响。
结果二黄胶囊可显著改善EAE的发病程度，降低血清MBP的含量，与模型对照组比较其差异有统计学意义（P<0.05）；二黄各剂量组大鼠大脑的2个取材部位、小脑、脑干炎性反应细胞浸润灶计数均明显少于模型对照组，差异具有统计学意义（P<0.01）；二黄各剂量组大脑和小脑、脑干的髓鞘病变灶计数均明显少于模型对照组，与模型对照组比较，差异有统计学意义（P<0.05）。二黄中剂量组脊髓纵切标本、二黄大、中剂量组脊髓横切标本的髓鞘病变灶计数明显少于模型对照组，与模型对照组比较，差异有统计学意义（P<0.05）。
结论二黄胶囊对EAE大鼠的体质量、动物发病情况和脑、脊髓病理改变具有明显改善作用。

关键词: 多发性硬化, 实验性变态反应性脑脊髓炎, 二黄胶囊

Abstract:

Objective To observe the protective and therapeutic effects of Erhuang capsule on experimental allergic encephalomyelitis(EAE) in rat model.
Methods Lewis rats were immunized by the mixture of antigen emulsion which contained spinal cord homogenate of guinea pig and complete Freund's adjuvant mixed in the ratio of 1:1 to induce EAE. The effects of Erhuang capsule on the incidence, CNS histopathologic change, and serum myelin basic protein level were observed.
Results Erhuang capsule could significantly ameliorate the clinical symptoms of EAE rats, reduce MBP content in serum, which were significantly different from those of the model group（P<0.05）. The infiltration areas of inflammatory cells in the brain, cerebellum, brain stem in different dosage groups of Erhuang capsule were significantly fewer than those in the model group（P<0.01）. The demyelination areas in the brain, cerebellum and brain stem in different dosage groups of Erhuang capsule were also significantly fewer than that in model group（P<0.05）. The demyelination areas in the longitudinal sections of spinal cord in the medium dose group of Erhuang capsule were notably fewer than those in the model group. The demyelination areas in the transections of spinal cord in the high and medium dose groups were also significantly fewer than that in the model group（P<0.05）.
Conclusion Erhuang capsule could increase the body weight of EAE rats, reduce the incidence, and cold also significantly improve the histopathologic change of brain and spinal cord.

Key words: multiple sclerosis, experimental allergic encephalomyelitis, Erhuang capsule

中图分类号:

R 282.71

李康宁;樊永平;陈克龙;周建平;邵燕;刘洪艳;杨文静. 二黄胶囊对实验性变态反应性脑脊髓炎模型大鼠急性期炎性反应和髓鞘修复的影响[J]. 首都医科大学学报, 2011, 32(1): 110-115.

LI Kang-ning;FAN Yong-ping;CHEN Ke-long;ZHOU Jian-ping;SHAO Yan;LIU Hong-yan;YANG Wen-jing. Effect of Erhuang Capsule on the Acute Inflammatory Reaction and Myelin Sheath Repairing in Model Rats of Experimental Allergic Encephalomyelitis[J]. Journal of Capital Medical University, 2011, 32(1): 110-115.

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