首都医科大学学报 ›› 2011, Vol. 32 ›› Issue (1): 17-20.

• 耳鼻咽喉头颈外科基础与临床研究 • 上一篇    下一篇

一氧化氮信号通路对小鼠鼻腔纤毛运动的调控

矫健1，王鸿1，金善哲1，张罗1，2*   

  1. 1. 教育部耳鼻咽喉头颈外科重点实验室，北京市耳鼻咽喉科研究所； 2. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-02-21 发布日期:2011-02-21
  • 通讯作者: 张罗

Regulatory Effect of Nitric Oxide Signal Pathway on Ciliary Motility in Mouse Nasal Epithelial Cells

JIAO Jian1, WANG Hong1, JIN Shan-zhe1, ZHANG Luo1,2*   

  1. 1. Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Institute of Otorhinolaryngology;2. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-21 Published:2011-02-21
  • Contact: ZHANG Luo

PDF

293

被引次数

4

摘要：

目的 观察一氧化氮（nitric oxide, NO）信号系统对小鼠鼻腔纤毛运动的调控。
方法 酶消化法培养小鼠鼻腔上皮细胞，采用高速数字化显微成像技术对纤毛摆动进行定量分析，观察10 mmol/L L-精氨酸（L-arginine，L-arg）对上皮细胞纤毛摆动的影响；采用免疫组化方法检测NO信号系统分子内皮型一氧化氮合酶（endothelial nitric oxide synthase，eNOS）、鸟苷酸环化酶（guanylate cyclase，GC）、蛋白激酶G（protein kinase G，PKG）的表达。
结果 ① Hanks平衡盐溶液（Hank’s balanced salt solution，HBSS）对照组小鼠鼻纤毛摆动在10 min内无明显变化；② 10 mmol/L L-arg处理组纤毛摆动频率（ciliary beat frequency，CBF）加药后2 min即出现明显增加，在检测的各个时间点，CBF与加药前相比差异有统计学意义；③ 免疫组化结果显示NO信号通路中主要信号分子eNOS、GC、PKG在小鼠鼻腔纤毛中均有明显表达。
结论 NO-环磷酸鸟苷(cyclic guanosine monophosphate，cGMP)-PKG信号通路参与了小鼠鼻纤毛运动的调控。

关键词: 一氧化氮, 信号通路, 精氨酸, 纤毛

Abstract:

Objective To investigate the effects of nitric oxide(NO) signal pathway on ciliary beat frequency(CBF) in mouse nasal epithelial cells.
Methods Cultured mouse nasal epithelial cells were treated with 10 mmol/L L-arginine, the CBF was measured by high-speed digital microscopy. In addition, expression of endothelial nitric oxide synthase(eNOS), guanylate cyclase(GC) and protein kinase G(PKG) were detected by immunohistochemistry.
Results ① CBF of epithelial cells in Hank’s balanced salt solution(HBSS) control group showed no significant changes in 10 min. ② CBF increased with treatment of 10 mmol/L L-arginine at 2 min and significant changes were observed at all the time points detected. ③ Immunoreactivity to eNOS, GC and PKG were observed in cilia of epithelial cells.
Conclusion NO-cGMP-PKG signal pathway participates in the regulating mechanism of ciliary motility in mouse nasal epithelial cells.

Key words: nitric oxide, signal pathway, arginine, cilia

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