5月龄APP/PS1双转基因小鼠行为学及老年斑病理改变

doi:10.3969/j.issn.1006-7795.2016.06.013

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (6): 784-788.doi: 10.3969/j.issn.1006-7795.2016.06.013

• 神经系统疾病的基础研究 • 上一篇下一篇

5月龄APP/PS1双转基因小鼠行为学及老年斑病理改变

陈晨, 杨翠翠, 李林, 张兰

首都医科大学宣武医院药物研究室北京市神经药物工程研究中心北京脑重大疾病研究院神经变性病教育部重点实验室, 北京 100053

收稿日期:2016-10-14 出版日期:2016-12-21 发布日期:2016-12-16
通讯作者: 张兰 E-mail:lanizhg@hotmail.com
基金资助:
国家自然科学基金（81473373），北京市自然科学基金（7132110），北京市卫生系统高层次卫生技术人才（2014-2-014），北京市教委新医药学科群（XK100270569）

Five-month-old APP/PS1 double transgenic mice behavior and pathology of senile plaques

Chen Chen, Yang Cuicui, Li Lin, Zhang Lan

Department of Pharmacology, Xuanwu Hospital, Capital Medical University, Beijing Engineering Research Center for Nerve System Drugs, Beijing Institute for Brain Disorders, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China

Received:2016-10-14 Online:2016-12-21 Published:2016-12-16
Supported by:
This study was supported by National Natural Science Foundation of China(81473373),Natural Science Foundation of Beijing (7132110),Beijing Health and Technical High-level Person Plan (2014-2-014),New Medical Disciplines Project of Beijing Education Committee(XK100270569).

摘要/Abstract

摘要： 目的评价5月龄APP/PS1双转基因小鼠学习记忆功能及脑部病理改变。方法 5月龄APP/PS1（+/-）小鼠，采用Morris水迷宫实验评价空间学习记忆功能，免疫组织化学和刚果红染色观察淀粉样斑块沉积情况。结果 Morris水迷宫实验显示APP/PS1（+）小鼠逃避潜伏期明显长于APP/PS1（-）小鼠，表明5月龄APP/PS1（+）小鼠已经出现了空间学习记忆功能的损害。新物体识别实验中5月龄APP/PS1（+）小鼠对新物体的分辨指数无显著降低，免疫组化、刚果红染色显示5月龄APP/PS1（+）小鼠海马及皮质均出现斑块沉积。结论 5月龄APP/PS1双转基因小鼠出现空间学习记忆功能损害，小鼠脑内已经出现相应的病理改变。

关键词: APP/PS1双转基因小鼠, β-淀粉样斑块, 刚果红染色

Abstract: Objective To explore behavior related to learning and memory ability of 5-month-old APP/PS1 double transgenic mice and pathology in the brain. Methods Five-month-old APP/PS1(+/-) double transgenic mice were tested with Morris water maze. β-amyloid plaques were studied with immunohistochemical staining and Congo red staining to observe-amyloid plaques deposition. ResultsAPP/PS1(+) mice escape latency was significantly longer than that of APP/PS1(-) mice, 5-month-oldAPP/PS1(+) mice learning and memory ability were damaged. β-amyloid plaques immunohistochemical staining and Congo red staining showed senile plaques in 5-month-old APP/PS1(+) mice. Conclusion Five-month-old APP/PS1 (+) mice learning and memory ability were damaged, a part of Alzheimer disease pathology was shown in the brain of 5-month-old APP/PS1(+) mice.

Key words: APP/PS1 double transgenic mouse, β-amyloid plaques, Congo red stain

中图分类号:

R592

陈晨, 杨翠翠, 李林, 张兰. 5月龄APP/PS1双转基因小鼠行为学及老年斑病理改变[J]. 首都医科大学学报, 2016, 37(6): 784-788.

Chen Chen, Yang Cuicui, Li Lin, Zhang Lan. Five-month-old APP/PS1 double transgenic mice behavior and pathology of senile plaques[J]. Journal of Capital Medical University, 2016, 37(6): 784-788.

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5月龄APP/PS1双转基因小鼠行为学及老年斑病理改变

Five-month-old APP/PS1 double transgenic mice behavior and pathology of senile plaques

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