七氟烷与高浓度氧对老年小鼠海马caspase-3的表达及认知功能的影响

doi:10.3969/j.issn.1006-7795.2017.03.004

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (3): 348-351.doi: 10.3969/j.issn.1006-7795.2017.03.004

七氟烷与高浓度氧对老年小鼠海马caspase-3的表达及认知功能的影响

陈玢¹, 刘伟¹, 史志国¹, 刘涛¹, 郑晖²

1. 首都医科大学附属北京胸科医院麻醉科, 北京 101149;
2. 国家癌症中心/中国医学科学院北京协和医学院肿瘤医院麻醉科, 北京 100021

收稿日期:2017-03-20 出版日期:2017-05-21 发布日期:2017-06-14
通讯作者: 郑晖 E-mail:chenghaocn@yahoo.com
基金资助:
国家自然科学基金（81371202）。

Impact of sevoflurane and high concentration of oxygen on expression of caspase-3 and cognition of aged mice

Chen Bin¹, Liu Wei¹, Shi Zhiguo¹, Liu Tao¹, Zheng Hui²

1. Department of Anesthesiology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China;
2. Department of Anesthesiology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China

Received:2017-03-20 Online:2017-05-21 Published:2017-06-14
Supported by:
This study was supported by National Natural Science Foundation of China(81371202).

摘要/Abstract

摘要： 目的探讨七氟烷与高浓度氧对老年小鼠海马半胱氨酸蛋白酶-3（caspase-3）的表达及认知功能的影响。方法健康雄性C57/BL6老年小鼠（14月龄）33只，采用数字表法将其随机分为3组：高浓度氧组（HO组，n=11）：吸100%（体积分数）氧4 h；高浓度氧+七氟烷组（HS组，n=11）：吸100%（体积分数）氧+2.5%（体积分数）七氟烷4 h；对照组（Con组，n=11）：吸空气4 h。24 h后采用Morris水迷宫法研究3组小鼠麻醉后连续5 d找到平台所用的时间（逃避潜伏期）和第5天去除平台后，90 s内穿过原平台的次数。利用蛋白质印记（Western blotting）法检测小鼠海马caspase-3的表达。结果与Con组相比，HO组的caspase-3表达差异无统计学意义（P>0.05）；HS组的穿越平台次数降低（P=0.023），caspase-3表达显著增加（P=0.036）。结论吸入100%（体积分数）氧对老年小鼠认知功能无影响，2.5%（体积分数）七氟烷与100%（体积分数）氧同时吸入增加老年小鼠海马caspase-3的表达，与老年小鼠产生认知功能障碍有关。

关键词: 老年小鼠, 认知功能障碍, 七氟烷, 氧气, 半胱氨酸蛋白酶

Abstract: Objective To investigate the impact of sevoflurane and high concentration of oxygen on expression of caspase-3 and cognitive function of aged mice. Methods Thirty three healthy male C57/BL6 elderly mice (14 months) were randomly divided into three groups:group of high concentration of oxygen (HO group, n=11):inhaling 100% oxygen for 4 h; high concentration of oxygen + sevoflurane group (HS group, n=11):taking in 100% oxygen with 2.5% sevoflurane for 4 h; control group (Con group, n=11):taking in air for 4 h. Cognitive function in the elderly mice was tested in the Morris water maze. Western blotting is used to test the expression of caspase-3 in hippocampus. Results Compared with Group Con, Group HO saw no difference in numbers of crossing the platform and Caspase-3 expression. Group HS crossed the platform less (P=0.023),and the caspase-3 expression of the group increased significantly(P=0.036).Conclusion Inhaling 100% oxygen had no impact on cognitive function in the elderly mice;Inhaling 2.5% Sevoflurane with 100% oxygen could increase expression of caspase-3, and it had close relations with the cognitive dysfunction of the aged mice.

Key words: elderly mice, cognitive dysfunction, sevoflurane, oxygen, caspase

中图分类号:

R614

陈玢, 刘伟, 史志国, 刘涛, 郑晖. 七氟烷与高浓度氧对老年小鼠海马caspase-3的表达及认知功能的影响[J]. 首都医科大学学报, 2017, 38(3): 348-351.

Chen Bin, Liu Wei, Shi Zhiguo, Liu Tao, Zheng Hui. Impact of sevoflurane and high concentration of oxygen on expression of caspase-3 and cognition of aged mice[J]. Journal of Capital Medical University, 2017, 38(3): 348-351.

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七氟烷与高浓度氧对老年小鼠海马caspase-3的表达及认知功能的影响

Impact of sevoflurane and high concentration of oxygen on expression of caspase-3 and cognition of aged mice

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