铜绿假单胞菌脂多糖对白色念珠菌生物膜的影响

doi:10.3969/j.issn.1006-7795.2017.04.019

摘要/Abstract

摘要： 目的研究铜绿假单胞菌脂多糖（lipopolysaccharide，LPS）对白色念珠菌生物膜形成的抑制作用。方法甲基四氮盐［（2，3-bis-（2-methoxy-4-nitro-5-sulphophenyl）-2H-tetrazolium-5-carboxanilide，XTT］减低法用于检测铜绿假单胞菌LPS对白色念珠菌生物膜形成不同阶段生成量的影响，同时，利用倒置显微镜观察生物膜形态学改变；实时定量聚合酶链式反应（quantitative real time polymerase chain reaction，qRT-PCR）法检测铜绿假单胞菌LPS对白色念珠菌生物膜菌丝特异基因（hypha specific genes，HSGs）表达的影响。结果铜绿假单胞菌LPS可以抑制白色念珠菌生物膜的形成，以及使菌丝特异基因HWP1、ALS1、ALS3、ECE1和SAP4的表达分别下调8.3~12.8、1.1~3.0、2.0~4.6、1.3~3.8和6.2~7.6倍。结论铜绿假单胞菌LPS可以通过抑制白色念珠菌菌丝的形成从而抑制其生物膜的形成。

关键词: 铜绿假单胞菌, 脂多糖, 白色念珠菌, 生物膜, 基因

Abstract: Objective To explore the inhibitory effects of Pseudomonas aeruginosa lipopolysaccharide on Candidas albicans biofilms. Methods ［(2,3-bis-(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide,XTT］ reduction assay was used to elucidate the effect of Pseudomonas aeruginosa lipopolysaccharide on biofilm formation of Candida albicans,and inverted microscope was used to observe the morphological changes of Candida albicans biofilms; expression analysis of Candida albicans hypha specific genes was done by quantitative real time polymerase chain reaction(PCR). Results The data showed that Pseudomonas aeruginosa lipopolysaccharide inhibits Candidas biofilm formation,and the expressions of HWP1, ALS1,ALS3,ECE1,SAP4 were downregulated 8.3 to 12.8 times,1.1 to 3.0 times,2.0 to 4.6 times,1.3 to 3.8 times and 6.2 to 7.6 times. Conclusion Pseudomonas aeruginosa lipopolysaccharide modulated Candida albicans biofilm formation by inhibiting the hyphal development.

Key words: Pseudomonas aeruginosa, lipopolysaccharide, Candidas albicans, biofilm, genes

中图分类号:

R379.4

邓娟, 孙伟, 马立艳, 苏建荣. 铜绿假单胞菌脂多糖对白色念珠菌生物膜的影响[J]. 首都医科大学学报, 2017, 38(4): 586-591.

Deng Juan, Sun Wei, Ma Liyan, Su Jianrong. Effects of Pseudomonas aeruginosa lipopolysaccharide on biofilm of Candida albicans[J]. Journal of Capital Medical University, 2017, 38(4): 586-591.

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