钛颗粒诱导骨溶解的不同动物模型形态学研究

doi:10.3969/j.issn.1006-7795.2021.01.007

首都医科大学学报 ›› 2021, Vol. 42 ›› Issue (1): 37-42.doi: 10.3969/j.issn.1006-7795.2021.01.007

钛颗粒诱导骨溶解的不同动物模型形态学研究

张晨¹, 刘子歌¹, 宋国瑞¹, 李燕², 陈德胜^3*, 程萌旗⁴

1.宁夏医科大学临床医学院,银川 750004;
2.宁夏医科大学基础医学院,银川 750004;
3.宁夏医科大学总医院骨科,银川 750004;
4.上海交通大学附属第六人民医院关节外科,上海 200011

收稿日期:2020-04-21 出版日期:2021-02-21 发布日期:2021-02-02
基金资助:
国家自然科学基金项目(81760405,81760395),宁夏回族自治区自然科学基金项目(2018AAC02013),宁夏医科大学校级课题(XZ2018014)。

Morphological study on different animal models of titanium particles inducing osteolysis

Zhang Chen¹, Liu Zige¹, Song Guorui¹, Li Yan², Chen Desheng^3*, Cheng Mengqi⁴

1. Ningxia Medical University Clinical Medical College,Yinchuan 750004,China;
2. Ningxia Medical University Basic Medical College,Yinchuan 750004,China;
3. Department of Orthopaedics, General Hospital of Ningxia Medical University,Yinchuan 750004,China;
4. Joint Surgery, The Sixth People's Hospital of Shanghai Jiao Tong University,Shanghai 200011,China

Received:2020-04-21 Online:2021-02-21 Published:2021-02-02
Contact: ^*E-mail:shuangshuangmama@163.com
Supported by:
National Natural Science Foundation of China(81760405,81760395),Natural Science Foundation of Ningxia Autonomous Region(2018AAC02013),School-level Project of Ningxia Medical University(XZ2018014).

摘要/Abstract

摘要： 目的通过钛颗粒诱导建立3种不同的骨溶解模型,比较其形态学差异、炎性反应水平、骨溶解情况以及破骨细胞计数等指标,为建立骨溶解动物模型的选择提供更好的依据。方法 45只雌性BALB/c小鼠采用数字表法随机分为钛颗粒刺激颅骨骨溶解模型(A组)、钛颗粒刺激气囊模型组(B组)及钛颗粒刺激气囊植骨模型组(C组)。通过大体观察和组织病理学对3组模型进行形态学观察;采用透射电子显微镜观察对比A、B两组骨溶解面积及C组软组织变化的超微结构,讨论其与人体标本病理生理变化的相似性。结果大体观察:A组可见颅骨表面有钛颗粒浸润,骨面不光整;B组气囊组织中有钛颗粒浸润,气囊囊壁增厚,有炎性水肿;C组可见颅骨块与周围的气囊组织之间有炎性细胞浸润,有钛颗粒包裹,且炎性反应明显。HE染色结果:A组颅骨表面钛颗粒周围有部分炎性反应,骨面欠平整;B组可见气囊囊壁厚,囊壁内有多种炎性细胞浸润,可有钛颗粒聚集;C组可见钛颗粒周围有炎性细胞浸润,骨片表面骨溶解表现。透射电子显微镜结果:A组破骨细胞胞质内见到钛颗粒,钛颗粒下有部分片状骨溶解;B组细胞胞质内有钛颗粒浸润,周围的细胞外散在有基质组织,基质组织中含水量减少;C组颅骨骨片骨面缺失,周围及与界膜之间有增生活跃的破骨细胞。结论 3种不同钛颗粒刺激下骨溶解动物模型的构建,形态学表现各有不同,可为假体周围骨溶解的基础研究提供有效的实验平台。

关键词: 动物模型, 骨溶解, 钛颗粒, 气囊

Abstract: Objective To establish three different osteolysis models induced by titanium particles, and to compare their morphological differences, inflammatory level, osteolysis and osteoclast count, so as to provide a better basis for the selection of osteolysis animal models. Methods Forty five female BALB/c mice were divided into three groups randomly: Titanium particles stimulate the skull osteolysis model (group A), titanium particles stimulate the balloon model group (group B) and titanium particles stimulate the balloon bone graft model group (group C).Morphologically observe three groups of models by gross observation and histopathology. The transmission electron microscope was used to observe and compare the osteolytic area of the group A and group B, and the ultrastructure of soft tissue changes in the group C. The similarities between them and the pathophysiological changes of human specimens were discussed. Results In general, there was infiltration of titanium particles on the surface of skull in group A, and the surface of bone was not smooth; there was infiltration of titanium particles, thickening of the wall of airbag, and inflammatory edema in the group B; there were infiltration of inflammatory cells between the skull block and the surrounding airbag tissue, wrapped by titanium particles, and the inflammatory reaction was obvious in group C. HE staining results: there were some inflammatory reactions around the titanium particles on the skull surface, and the bone surface was not smooth in group A; there were many kinds of inflammatory cells infiltrated in the cyst wall, and titanium particles could gather in group B; there were inflammatory cells infiltrated around the titanium particles, and osteolysis on the bone surface was seen in group C. By transmission electron microscope: titanium particles were found in the cytoplasm of osteoclasts in group A, and some lamellar osteolysis was found under the titanium particles; titanium particles were infiltrated in the cytoplasm of cells in group B, matrix tissue was scattered around the cells, and the water content in the matrix tissue was reduced; osteoclast precursor cells were proliferative and active around the bone tissue in group C, loss of bone surface. Conclusion Three kinds of animal models of osteolysis stimulated by titanium particles have different morphological manifestations, which can provide an effective experimental platform for the basic research of osteolysis around prosthesis.

Key words: animal model, osteolysis, titanium particles, air pouch

中图分类号:

R9336

张晨, 刘子歌, 宋国瑞, 李燕, 陈德胜, 程萌旗. 钛颗粒诱导骨溶解的不同动物模型形态学研究[J]. 首都医科大学学报, 2021, 42(1): 37-42.

Zhang Chen, Liu Zige, Song Guorui, Li Yan, Chen Desheng, Cheng Mengqi. Morphological study on different animal models of titanium particles inducing osteolysis[J]. Journal of Capital Medical University, 2021, 42(1): 37-42.

参考文献

[1] 李睿,陈继营.谈谈关节假体周围感染的诊断问题[J].中国骨与关节杂志,2019,8(4):241-248.
[2] Yoshimi E, Christian G, William T, et al. Imaging evaluation of polyethylene liner dissociation in total hip arthroplasty[J]. Skeletal Radiol, 2019,48(12):1933-1939.
[3] Cao X P, Fang X Y, Malik W S, et al. TRB3 interacts with ERK and JNK and contributes to the proliferation, apoptosis, and migration of lung adenocarcinoma cells[J].J Cell Physiol,2020,235(1):538-547.
[4] Santul B,Daniel H, Akul M, et al.Pros and cons of mouse models for studying osteoarthritis[J]. Clin Translat Med,2018,7(1):36.
[5] Jukka P H,Tzu-Hua L, Akira N, et al. Mesenchymal stem cells in the aseptic loosening of total joint replacements[J]. J Biomed Mater Res A,2017,105(4):1195-1207.
[6] Allen M,Brett F,Millett P,et al.ne effects of particulate polyethylene at a weight-beating bone-implant interface:a study in rats[J].J Bone Joint Surg Br,1996, 78(1):32-37.
[7] 张晨,李燕,郭凤英,等.磨损颗粒刺激小鼠air pouch气囊建立骨溶解动物模型的实验研究[J].宁夏医学杂志,2019,41(7):577-580.
[8] Kyeong M S, Hyun A J, Jeong I H, et al.Development of a mouse model of knee osteoarthritis based on obesity and bipedal walking[J].J Orthop Res,2019,37(11):2411-2419.
[9] Ren W. Macrophage depletion diminishes implant-wear-induced inflammatory osteolysis in a mouse model[J]. J Biomed Mater Res A, 2008, 85(4):1043-1051.
[10]陈德胜,李燕,郭凤英,等.钛颗粒刺激小鼠颅骨诱导骨溶解的动物模型建立[J].宁夏医学杂志,2018,40(8):677-680.
[11]赵江博,李燕,田佳宁,等.扫描电子显微镜下观察磨损颗粒刺激小鼠气囊植骨模型诱导骨溶解的超微病理改变[J].宁夏医学杂志,2019,41(3):211-213.
[12]Qu R Z, Chen X M, Yuan Y J, et al. Ghrelin fights against titanium particle-induced inflammatory osteolysis through activation of β-catenin signaling pathway[J].Inflammation,2019,42(5):1652-1665.
[13]Liao S J, Song F M, Feng W Y, et al.Rhoifolin ameliorates titanium particle-stimulated osteolysis and attenuates osteoclastogenesis via RANKL-induced NF-κB and MAPK pathways[J].J Cell Physiol,2019,234(10):17600-17611.
[14]Khaled H S, Norbert L,Markus T,et al. Severe metallosis-related osteolysis as a cause of failure after total knee replacement[J]. J Clin Orthop Trauma,2020,11(1): 1022-1031.
[15]王伟,周胜虎,刘军,等.磨损颗粒与人工关节骨溶解的相关研究[J].中国骨与关节损伤杂志,2017,32(4):446-448.
[16]Wei G J, Liang T H, Wei C M,et al. Arctigenin inhibits RANKL-induced osteoclastogenesis and hydroxyapatite resorption in vitro and prevents titanium particle-induced bone loss in vivo[J].J Cell Biochem, 2019,120(4):5367-5376.
[17]Park Y H, Kim H J.Combination of gp130-targeting and TNF-targeting small molecules in alleviating arthritis through the down-regulation of Th17 differentiation and osteoclastogenesis[J].Biochem Biophys Res Commun, 2020, 522 (4): 1030-1036.
[18]Li C, Wu Z X, Yuan G X, et al.Vx-11e protects against titanium-particle-induced osteolysis and osteoclastogenesis by supressing ERK activity[J].Biochem Biophysi Res Commun, 2019,514(4):1244-1250.
[19]Chen P,Tang T, Liu C L, et al.High-temperature requirement a1 protease as a rate-limiting factor in the development of osteoarthritis[J]. Am J Pathol,2019,189(7):1423-1434.
[20]赵江博,李燕,田佳宁,等.磨损颗粒诱导骨溶解的两种动物模型形态学比较研究[J].实用骨科杂志,2019,25(1):44-47.
[21]Li S X, Qiu J X, Qin L, et al. NOD2 negatively regulated titanium particle-induced osteolysis in mice[J].Biomater Sci,2019,7(7):2702-2715.
[22]Yang G P, Gu M H, Chen W H, et al. SPHK-2 promotes the particle-induced inflammation of RAW264.7 by maintaining consistent expression of TNF-α and IL-6[J].Inflammation,2018,41(4):1498-1507.

钛颗粒诱导骨溶解的不同动物模型形态学研究

Morphological study on different animal models of titanium particles inducing osteolysis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	黄琪, 周亦伦, 孙雪峰. 共享外周循环对糖尿病肾病小鼠肾损伤的影响[J]. 首都医科大学学报, 2022, 43(5): 674-679.
[2]	李妍. 守护“记忆之魂”的中国痴呆临床研究引领者——贾建平教授[J]. 首都医科大学学报, 2020, 41(5): 822-827.
[3]	刘鑫垚, 马超, 贾竑晓. 精神分裂症认知损害动物模型的研究现状[J]. 首都医科大学学报, 2020, 41(1): 50-54.
[4]	马登磊, 张旭, 罗艺, 黄蕊, 李雅莉, 李林, 张兰. 微管相关蛋白tau转基因细胞和动物模型的建立及tau蛋白病变表征[J]. 首都医科大学学报, 2019, 40(4): 582-587.
[5]	王佳, 乐凯, 郭懿樊, 郭旭, 张涛, 任宇, 王睿斌, 许光中, 郑海亮, 冯树理, 朱斌. 同步辐射X线相衬成像对裸鼠胰腺癌的早期诊断[J]. 首都医科大学学报, 2019, 40(2): 195-200.
[6]	杨硕, 曹金鑫, 王明刚, 卢静, 陈杰, 申英末, 邹振玉, 刘雨辰. 去腹壁运动神经支配建立兔腹壁薄弱模型[J]. 首都医科大学学报, 2019, 40(2): 282-285.
[7]	邹振玉, 杨硕, 王明刚, 曹金鑫, 钟杏霞, 申英末, 陈杰. 新型巨大腹壁疝及腹腔高压动物模型的实验研究[J]. 首都医科大学学报, 2018, 39(6): 900-904.
[8]	肖鲁瑶, 张杰, 郑帅, 梅少帅. pH值和剂量对腹腔注射L-精氨酸诱导小鼠急性胰腺炎模型的影响[J]. 首都医科大学学报, 2017, 38(5): 700-703.
[9]	谭赛, 雷小明, 王彦云, 潘菊华, 张颖, 黄世敬, 张永超, 郑军, 陈朝, 陈宇霞, 李多娇. 基于慢性缺血应激建立血管性抑郁症动物模型[J]. 首都医科大学学报, 2016, 37(2): 196-202.
[10]	任宇, 侯晓朴, 李丹, 李颖, 朱斌. 小剂量DMBA对大鼠胰腺的致瘤作用[J]. 首都医科大学学报, 2015, 36(6): 936-941.
[11]	刘洁;王泳;武连华;高春锦. 高压氧预处理对小鼠低氧耐受能力的影响[J]. 首都医科大学学报, 2011, 32(5): 670-673.
[12]	张洪海;孙玉;柳雅立;张彤;吴昊;陈德喜. gp120转基因小鼠制备及初步鉴定[J]. 首都医科大学学报, 2010, 31(6): 695-700.
[13]	马力;刘杰;初楠;杨进刚;史旭波;王雅杰;李田昌;胡大一. 心力衰竭模型大鼠心脏肥厚指标与心功能的关系研究[J]. 首都医科大学学报, 2010, 31(5): 596-599.
[14]	王文峰;汪欢;钱伟;侯晓华. 急性肠道旋毛虫感染后小鼠和大鼠内脏敏感性变化比较[J]. 首都医科大学学报, 2010, 31(3): 315-319.
[15]	任添华;张淑文. 中药复方912注射液对MODS动物模型细胞因子的实验研究[J]. 首都医科大学学报, 2010, 31(2): 251-254.