首都医科大学学报 ›› 2024, Vol. 45 ›› Issue (1): 104-110.doi: 10. 3969/ j. issn. 1006-7795. 2024. 01. 017

• 基础研究 • 上一篇    下一篇

一氧化氮供体型药物对牙龈卟啉单胞菌体外抗菌活性的影响

江玉梅1,张紫薇2,王吉天2,杨宇2*   

  1. 1.京东口腔医院综合科, 北京 101200;2.首都医科大学附属北京朝阳医院口腔科, 北京 100043
  • 收稿日期:2023-09-04 出版日期:2024-02-21 发布日期:2024-03-22
  • 通讯作者: 杨宇 E-mail:13671027896@163.com

Effect of nitric oxide donor drugs on the antibacterial activity of Porphyromonas gingivalis in vitro

Jiang Yumei1,Zhang Ziwei2,Wang Jitian2,Yang Yu2*   

  1. 1.Comprehensive Department of JD Stomatological Hospital,Beijing 101200, China ;2.Department of Stomatology,Beijing Chaoyang Hospital,Capital Medical University, Beijing 100043,China
  • Received:2023-09-04 Online:2024-02-21 Published:2024-03-22

摘要: 目的  研究一氧化氮(nitric oxide,NO)供体型药物对牙龈卟啉单胞菌(Porphyromonas gingivalisP.g)体外抗菌活性的影响。方法  采用菌落计数法、总NO检测试剂盒、活性氧检测试剂盒、钙离子(Ca2+)荧光探针Fluo-4 AM检测不同浓度的NO(10、30、50 mmol/L)对P.g的体外抗菌性能,以及对P.g胞内活性氧(reactive oxygen species,ROS)水平和Ca2+浓度的影响。结果  NO对P.g有显著的体外抗菌活性,并且其抗菌活性与NO药物浓度和作用时间具有明显的正相关性(P<0.05)。NO处理后显著提高了P.g胞内ROS水平和Ca2+浓度(P<0.05)。结论  NO能够有效抑制P.g的活性和生长能力,并引起P.g胞内ROS和Ca2+代谢紊乱。NO供体型药物对种植体周围炎具有潜在的治疗作用和临床应用价值。

关键词: 一氧化氮供体型药物, 牙龈卟啉单胞菌, 抗菌作用, 种植体周围炎, 活性氧, 钙离子

Abstract: Objective  To study the effect of nitric oxide (NO) on the in vitro antibacterial activity of Porphyromonas gingivalis (P.g). Methods  The antibacterial performance of different concentrations of NO (10,30,50 mmol/L) on P.g was assessed by colony forming units,total NO detection kit,reactive oxygen species detection kit,and calcium ion fluorescent probe Fluo-4 AM. Moreover,the effects of NO treatment on intracellular levels of reactive oxygen species (ROS) and calcium ions in P.g cells were also examined.  Results  NO exhibited significant in vitro antibacterial activity against in P.g cells,and its antibacterial activity was positively correlated with the concentration of NO drugs and treatment duration (P<0.05). NO treatment significantly increased intracellular levels of ROS and calcium ions in P.g cells (P<0.05). Conclusion  NO can effectively inhibit the activity and growth ability of P.g,and cause intracellular ROS and calcium ion metabolism disorders. Our study demonstrates the potential therapeutic effect and clinical application of NO donor drugs in peri-implantitis.

Key words: nitric oxide donor drugs, Porphyromonas gingivalis (P.g), antimicrobial activity, peri-implantitis, reactive oxygen species, Ca2+

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