姜黄素调控TRIM2介导mTOR通路抑制RKO细胞增殖的机制研究

doi:10.3969/j.issn.1006-7795.2025.05.013

首都医科大学学报 ›› 2025, Vol. 46 ›› Issue (5): 845-852.doi: 10.3969/j.issn.1006-7795.2025.05.013

姜黄素调控TRIM2介导mTOR通路抑制RKO细胞增殖的机制研究

于航^1,2,武海阔^1,2,赵千慧^1,2,李宇栋^3*,刘剑^1,2

1.天津中医药大学第一附属医院肛肠科，天津 300381；2.国家中医针灸临床医学研究中心，天津 300381；3.首都医科大学附属北京中医医院肛肠科，北京 100010

收稿日期:2025-01-23 修回日期:2025-06-24 出版日期:2025-10-21 发布日期:2025-10-22
通讯作者: 李宇栋 E-mail:liyudong@bjzhongyi.com
基金资助:
中华中医药学会（2022~2024年度）青年人才托举工程项目（CACM-2022-QNRC2-A03），中华中医药学会雏鹰计划中医临床青年人才研修项目（CYJH2024024），北京市医院管理中心创新梦工场经费资助（202122）。

Curcumin suppresses RKO cell proliferation by targeting TRIM2-mediated regulation of the mTOR signaling pathway

Yu Hang^1,2, Wu Haikuo^1,2, Zhao Qianhui^1,2, Li Yudong^3*, Liu Jian^1,2

1.Department of Colorectal Surgery, the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China;2.National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China;3.Department of Colorectal Surgery, Beijing Hospital of Traditional Chinese Medicine, Capital Medical Univetsity, Beijing 100010, China

Received:2025-01-23 Revised:2025-06-24 Online:2025-10-21 Published:2025-10-22

摘要/Abstract

摘要： 目的本研究旨在探讨姜黄素对结肠癌RKO细胞增殖的抑制作用及其机制，重点关注三结构域蛋白2（tripartite motif-containing protein 2，TRIM2）表达和哺乳动物雷帕霉素靶蛋白（mammalian target of rapamycin, mTOR）信号通路的调控。方法通过分析计算半数抑制浓度（half maximal inhibitory concentration, IC50）值，确定实验姜黄素给药浓度。采用实时荧光定量聚合酶链式反应（quantitative polymerase chain reaction, qPCR）法检测RKO细胞和人正常肠黏膜细胞（fetal human colon, FHC）中TRIM2表达水平。通过蛋白质免疫印迹实验（Western blotting, WB）检测姜黄素处理后RKO细胞中TRIM2及敲减TRIM2后RKO细胞mTOR通路相关蛋白表达情况。利用细胞计数试剂盒（cell counting kit-8, CCK8）检测姜黄素处理、TRIM2敲减及mTOR信号通路激活剂对RKO细胞增殖的影响。结果 TRIM2在RKO细胞中异常高表达。姜黄素可降低RKO细胞TRIM2表达，敲减TRIM2后RKO细胞mTOR信号通路相关蛋白表达下调。姜黄素和TRIM2敲减均显著抑制RKO细胞增殖，而mTOR信号通路激活剂可逆转这种抑制作用。结论姜黄素通过下调TRIM2表达调控mTOR信号通路抑制结肠癌RKO细胞增殖。

关键词: 姜黄素, 结肠癌, TRIM2, mTOR, 信号通路, 细胞增殖

Abstract: Objective The study aims to elucidate the inhibitory effects of curcumin on the proliferation of colon cancer RKO cells, focusing on the regulatory mechanisms of tripartite motif-containing protein 2(TRIM2) expression and the mammalian target of rapamycin(mTOR) signaling pathway. Methods Experimental concentrations of curcumin were determined by calculating the half maximal inhibitory concentration(IC50) value. Quantitative polymerase chain reaction(qPCR) was utilized to assess the level of TRIM2 expression in RKO and fetal human colon(FHC) cells. Western blotting analysis was conducted to investigate the level of TRIM2 expression and mTOR pathway-related proteins in curcumin-treated RKO cells. The impact of curcumin treatment, TRIM2-knockingdown, and mTOR signaling pathway on proliferation in RKO cells was quantified using the cell counting kit-8(CCK8) assay. Results The expression of TRIM2 was found to be elevated in RKO cells as determined by qPCR, compared to FHC cells. Curcumin suppressed the level of TRIM2 expression, and subsequent knockdown of TRIM2 resulted in decreased expression of mTOR-related proteins in RKO cells. Both curcumin and TRIM2-knockdown demonstrated significant inhibition of proliferation in RKO cells, with reversion upon activation of mTOR signaling pathway. Conclusion The study unveils the inhibitory effects of curcumin on RKO cells proliferation through modulation of TRIM2 expression and the mTOR signaling pathway.

Key words: curcumin, colon cancer, TRIM2, mTOR, signaling pathway, cell proliferation

中图分类号:

R735.3

于航, 武海阔, 赵千慧, 李宇栋, 刘剑. 姜黄素调控TRIM2介导mTOR通路抑制RKO细胞增殖的机制研究[J]. 首都医科大学学报, 2025, 46(5): 845-852.

Yu Hang, Wu Haikuo, Zhao Qianhui, Li Yudong, Liu Jian. Curcumin suppresses RKO cell proliferation by targeting TRIM2-mediated regulation of the mTOR signaling pathway[J]. Journal of Capital Medical University, 2025, 46(5): 845-852.

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姜黄素调控TRIM2介导mTOR通路抑制RKO细胞增殖的机制研究

Curcumin suppresses RKO cell proliferation by targeting TRIM2-mediated regulation of the mTOR signaling pathway

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