关键词: α-突触核蛋白, 帕金森病, 血清, 寡聚体

Abstract: Objective To study the oligomerization of α-Synuclein (α-Syn) in the sera of patients with Parkinson's disease (PD) and the potential mechanism for this oligomerization. Methods Recombinant α-Syn was incubated in the sera of PD patients and of normal subjects. Immunoblot analysis was used to identify the formation of α-Syn oligomers. A special ELISA method was used to evaluate the amount of α-Syn oligomers in PD and control sera. Results The oligomeric forms of α-Syn formed in the serum were dimers, trimers, tetramers, and fourteen-polymers. Compared with control sera, the sera of PD patients significantly enhanced the oligomerization of α-Syn. Reduced glutathione significantly decreased the amount of α-Syn oligomers in the sera of PD patients. Conclusion α-Syn can form different forms of oligomers in the serum. Compared with control, sera from PD patients enhance the oligomerization of α-Syn, which may be mediated by oxidization of α-Syn.

Key words: α-Synuclein, Parkinson's disease, serum, oligomer