APP17肽对卵巢去势大鼠海马神经元凋亡相关因子表达的影响

首都医科大学学报 ›› 2001, Vol. 22 ›› Issue (4): 296-299.

APP17肽对卵巢去势大鼠海马神经元凋亡相关因子表达的影响

谭宁, 赵咏梅, 赵志炜, 姬志娟, 盛树力

首都医科大学宣武医院脑老化实验室

收稿日期:2001-06-20 修回日期:1900-01-01 出版日期:2001-10-15 发布日期:2001-10-15

Effect of APP17-mer Peptide on Expression of Apoptosis Related Proteins in Hippocampal Neurons of Ovariectomized Rats

Tan Ning, Zhao Yongmei, Zhao Zhiwei, Ji Zhijuan, Sheng Shuli

Beijing Research Laboratory for Brain Aging, Xuanwu Hospital, Affiliate of Capital University of Medical Sciences

Received:2001-06-20 Revised:1900-01-01 Online:2001-10-15 Published:2001-10-15

摘要/Abstract

摘要： 为研究卵巢去势大鼠海马神经元内凋亡相关因子Fas、Fas-L、NFκB、c-fos、c-Jun的表达及APP17肽对这些因子表达的影响,将健康雌性Wistar大鼠行双侧卵巢切除手术造模,并用APP17肽治疗,分别用免疫组化方法观察Fas、Fas-L、NFκB、c-fos、c-Jun的表达,用TUNEL方法检测神经元的凋亡.结果发现:卵巢去势大鼠海马神经元Fas、Fas-L、c-fos、c-Jun的表达增高,而NFκB的表达则减少;使用APP17肽治疗后,上述蛋白质的表达恢复到正常水平,TUNEL检测未发现凋亡神经元.提示卵巢去势大鼠发生了海马神经元的凋亡相关蛋白的改变,可能是神经元处于凋亡前状态;APP17肽可改善卵巢去势大鼠海马神经元内凋亡相关蛋白的表达,维持神经元的正常功能.

关键词: 卵巢去势, 凋亡, APP17肽

Abstract: To study the expressions of apoptosis related proteins Fas, Fas-L, NFκB, c-fos and c-Jun in hippocampal neurons of ovariectomized rats, and the effect of APP17-mer peptide on these expressions, female Wistar rats were bilaterally ovariectomized(ovx), and were treated with APP17-mer peptide. Then they were studied by immunohistochemistry for Fas, Fas-L, c-fos, c-Jun and NFκB, and were studied by TUNEL to observe the DNA fragments of neurons. The number of neurons stained positively by Fas, Fas-L, c-fos, c-Jun antibodies in the brains of ovariectomized rats increased, and the number of neurons stained positively for NFκB was reduced. However, after being treated by the APP17-mer peptide, the expression of these apoptosis related proteins were normalized. There were no apoptotic neurons stained by TUNEL in the hippocampus. The result indicates that expression changes of apoptotic related protein in OVX rats. APP17-mer peptide treatment can normalize the expression of these proteins mentioned above. According to the research, the hippocampal neurons appear in a condition of pro apoptosis, but APP17-mer peptide is able to reverse the pro apoptosis situation of neurons.

Key words: ovariectomy, apoptosis, APP17P

中图分类号:

R 338.1

谭宁;赵咏梅;赵志炜;姬志娟;盛树力. APP17肽对卵巢去势大鼠海马神经元凋亡相关因子表达的影响[J]. 首都医科大学学报, 2001, 22(4): 296-299.

Tan Ning;Zhao Yongmei;Zhao Zhiwei;Ji Zhijuan;Sheng Shuli . Effect of APP17-mer Peptide on Expression of Apoptosis Related Proteins in Hippocampal Neurons of Ovariectomized Rats[J]. Journal of Capital Medical University, 2001, 22(4): 296-299.

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