首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (4): 566-571.doi: 10.3969/j.issn.1006-7795.2013.04.017

• 基础研究 • 上一篇    下一篇

双侧黑质注射脂多糖引起大鼠迷走神经背核胆碱能标志物降低和便秘

张悦, 郑丽飞, 宋瑾, 樊瑞芳, 陈长亮, 朱进霞   

  1. 首都医科大学基础医学院生理与病理生理学系, 北京 100069
  • 收稿日期:2013-02-21 出版日期:2013-08-21 发布日期:2013-07-20
  • 通讯作者: 郑丽飞 E-mail:zhenglifei001@yahoo.cn
  • 基金资助:

    国家自然科学基金(81170346);北京市自然科学基金(7121003,7132017)。

Reduced expression of cholinergic neuronal marker in the dorsal motor nucleus of the vagus and constipation are observed in the rats with lipopolysaccharide microinjection in bilateral substantia nigra

ZHANG Yue, ZHENG Lifei, SONG Jin, FAN Ruifang, CHEN Changliang, ZHU Jinxia   

  1. Department of Physiology and Pathophysiology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China
  • Received:2013-02-21 Online:2013-08-21 Published:2013-07-20
  • Supported by:

    This study was supported by National Natural Science Foundation of China(81170346), Natural Science Foundation of Beijing(7121003, 7132017).

摘要:

目的 黑质内注射脂多糖(lipopolysaccharide,LPS)制备帕金森模型大鼠(LPS大鼠),观察LPS大鼠排便情况及迷走运动背核(dorsal motor nucleus of the vagus,DMV)中酪氨酸羟化酶(tyrosine hydroxylase,TH)和胆碱乙酰转移酶(choline acetyltransferase,ChAT)免疫反应阳性神经元胞体及神经纤维的分布特点。方法 通过双侧黑质内注射LPS 获得LPS大鼠;免疫荧光组织化学用于检测TH和ChAT免疫阳性神经元在DMV的分布。结果 LPS大鼠摄食和饮水情况与对照组相比较,差异无统计学意义,但粪便湿质量减轻,粪便内含水量显著减少,即出现便秘情况。LPS大鼠黑质内神经元大量丢失,并伴有胶质细胞增生、TH免疫阳性(IR)明显降低;而DMV内的TH-IR显著增强、ChAT-IR明显减弱。结论 与黑质内注射六羟多巴相似,黑质内注射LPS可以引起大鼠出现便秘,该结果可能与LPS大鼠DMV中TH增多和ChAT降低导致胃肠动力紊乱有关。

关键词: 脂多糖, 帕金森模型, 便秘, 迷走运动背核, 胆碱乙酰转移酶

Abstract:

Objective To study the alteration of the distribution of tyrosine hydroxylase(TH) and choline acetyltransferase(ChAT) immunoreactivity-positive neurons and nerve fibers in the dorsal motor nucleus of the vagus(DMV) in rats with lipopolysaccharide(LPS) microinjection of substantia nigra(SN). Methods Rats were subjected to LPS in bilateral SN to make the LPS Parkinson's disease rat model(LPS rats). Immunofluorescence were used in this study. Results In the rats treated with LPS, the neurons' number in SN was markedly reduced accompanied by abundant proliferation of glial cells, and the TH immunoreactivity(IR) in SN was obviously reduced. However, the TH-IR neurons were significantly increased, and the ChAT-IR significantly reduced in DMV. Furthermore, the rats treated with LPS showed obvious constipation with reduced weight and water content in the stool. Conclusion Injection of LPS into substantia nigra can cause a constipation in rats, which may be related to the enhanced TH-IR and reduced ChAT-IR in DMV leading to gastrointestinal dysmotility in LPS treated rats.

Key words: lipopolysaccharide, Parkinson’s disease model, constipation, dorsal motor nucleus of the vagus, choline acetyltransferase

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