HIV-1膜蛋白gp120 V3环的神经毒性研究

doi:10.3969/j.issn.1006-7795.2014.01.019

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (1): 87-91.doi: 10.3969/j.issn.1006-7795.2014.01.019

• 艾滋病基础与临床研究 • 上一篇下一篇

HIV-1膜蛋白gp120 V3环的神经毒性研究

张志丽¹, 陈兵刚², 姜太一¹, 乔录新³, 吴昊¹, 陈德喜³, 张玉林¹

1. 首都医科大学附属北京佑安医院感染中心, 北京 100069;
2. 山东省滨州市中心医院感染内科, 山东滨州 251700;
3. 北京市肝病研究所, 北京 100069

收稿日期:2013-12-03 出版日期:2014-02-21 发布日期:2014-02-21
通讯作者: 张玉林 E-mail:13051222056@126.com
基金资助:
国家自然科学基金（81371399，81272266）；“十二五”国家科技支撑计划重大项目（2012ZX10001-002，2012ZX10001-003，2012ZX10001-004）；北京市艾滋病研究重点实验室（BZ0089）。

Neurotoxicity of HIV-1 envelope protein gp120 V3 loop

Zhang Zhili¹, Chen Binggang², Jiang Taiyi¹, Qiao Luxin³, Wu Hao¹, Chen Dexi³, Zhang Yulin¹

1. Department of Infectious Diseases, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China;
2. Department of Infectious Diseases, Binzhou Central Hospital, Binzhou 251700, Shandong Province, China;
3. Beijing Institute of Hepatology, Beijing 100069, China

Received:2013-12-03 Online:2014-02-21 Published:2014-02-21
Supported by:
This Study was Supported by the National Natural Science Foundation of China (81371399, 81272266);Key Projects of the National infectious diseases special research fund during the Twelfth Five-year Research Program of China (2012ZX10001-002, 2012ZX10001-003, 2012ZX10001-004);Beijing Key Laboratory of AIDS (BZ0089).

摘要/Abstract

摘要：

目的体外观察人类免疫缺陷病毒-1（human immunodeficiency virus-1，HIV-1）膜蛋白gp120 V3环的神经毒性。方法通过小鼠大脑皮质原代神经细胞培养条件下TUNEL检测和培养上清乳酸脱氢酶释放试验，结合抗微管蛋白-2（microtubule associated protein-2，MAP-2）免疫荧光试验，观察人重组gp120蛋白神经毒性，并通过蛋白印迹法初步探讨其与凋亡调控蛋白bax与bcl-2的关系。结果重组人gp120 V3环多肽可诱导神经元凋亡和抑制神经突起形成，并呈浓度依赖性。gp120 V3环诱导神经细胞凋亡与抑制bcl-2表达有关。结论 HIV-1膜蛋白gp120 V3环具有神经毒性。

关键词: 膜蛋白gp120, 神经毒性, V3环, 凋亡

Abstract:

Objective To investigate the neurotoxicity of human immunodeficiency virus-1(HIV-1) envelope protein gp120 in vitro. Methods Primary mouse cortical neuronal cultures were firstly established for neurotoxicity analysis. Terminal dUTP nick end-labeling (TUNEL) assay and lactate dehydrogenase (LDH) leakage assay were used to analyze neuronal apoptosis induced by recombinant gp120 V3 loop polypeptide. Further, Western blotting was used to detect the levels of apoptosis associated proteins bcl-2 and bax under gp120 V3 loop exposure. Results Recombinant gp120 V3 loop polypeptide was found to induce neuronal apoptosis and inhibit neurite outgrowth in a concentration dependent manner, which was associated with low expression of bcl-2 proteins. Conclusion HIV-1 envelope protein gp120 has neurotoxicity.

Key words: envelope protein gp120, neurotoxicity, V3 loop, apoptosis

中图分类号:

R512.91

张志丽, 陈兵刚, 姜太一, 乔录新, 吴昊, 陈德喜, 张玉林. HIV-1膜蛋白gp120 V3环的神经毒性研究[J]. 首都医科大学学报, 2014, 35(1): 87-91.

Zhang Zhili, Chen Binggang, Jiang Taiyi, Qiao Luxin, Wu Hao, Chen Dexi, Zhang Yulin. Neurotoxicity of HIV-1 envelope protein gp120 V3 loop[J]. Journal of Capital Medical University, 2014, 35(1): 87-91.

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HIV-1膜蛋白gp120 V3环的神经毒性研究

Neurotoxicity of HIV-1 envelope protein gp120 V3 loop

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