重度宫腔粘连相关基因的生物信息学分析

doi:10.3969/j.issn.1006-7795.2018.05.017

首都医科大学学报 ›› 2018, Vol. 39 ›› Issue (5): 715-720.doi: 10.3969/j.issn.1006-7795.2018.05.017

重度宫腔粘连相关基因的生物信息学分析

柳鑫, 段华, 成九梅, 陈芳, 徐倩

首都医科大学附属北京妇产医院妇科微创中心, 北京 100006

收稿日期:2018-04-03 出版日期:2018-09-21 发布日期:2018-10-20
通讯作者: 段华 E-mail:duanhuasci@163.com
基金资助:
首都医科大学科研培育基金（PYZ2017004）。

Bio-informative analysis of severe intrauterine adhesion related gene

Liu Xin, Duan Hua, Cheng Jiumei, Chen Fang, Xu Qian

Mini-invasive Center, Beijing Obstetric and Gynecology Hospital, Capital Medical University, Beijing 100006, China

Received:2018-04-03 Online:2018-09-21 Published:2018-10-20
Supported by:
This study was supported by Capital Medical University Scientific Research Cultivation(PYZ2017004).

摘要/Abstract

摘要： 目的从分子水平探讨重度宫腔粘连（intrauterine adhesion，IUA）的发生机制。方法重度宫腔粘连组织及正常子宫组织每组13例标本，各组取3例用于芯片分析，10例用于RT-PCR验证芯片结果。应用芯片检测两组中差异表达的基因，采用GO数据库及京都基因与基因组大百科全书数据库（Kyoto Encyclopedia of Genes and Genomes，KEGG）对差异表达的基因进行基因功能分析与信号传导通路分析（pathway analysis）。结果筛选出1 425个差异基因，其中上调基因731个，下调基因694个，对其进行生物信息学分析发现，差异表达基因功能主要包括：信号传导、多种细胞有机体发育、细胞粘连、细胞分化、单核细胞活化等。对部分差异表达基因COL16A1、ADAM-9，CDH2、LOX进行RT-PCR验证，结果表明其表达趋势与芯片结果一致。结论利用生物信息学分析能有效挖掘基因芯片内在数据，对部分差异表达基因进一步分析表明，宫腔粘连发生发展中可能存在通过调节细胞外基质的表达与功能的机制，调节宫腔粘连的形成。

关键词: 宫腔粘连, 基因芯片, 生物信息学

Abstract: Objective To detect the mechanism in molecular level of severe intrauterine adhesion(IUA). Methods Thirteen severe IUA tissues and thirteen normal uterus tissues were abtained.Three samples of each group were tested by gene chip.Ten remained samples of each group were used to verified the results of the gene chip by RT-PCR. Then the target genes were subjected to gene ontology and pathway analysis by Go database and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results A total of 1 425 mRNA species were differentially expressed in IUA tissue versus normal uterineus tissue. Of these, 731 mRNAs were highly expressed and 694 mRNAs were lightly minimally expressed in IUA tissues. Further functional analysis showed the function of these gene are involved in signal transduction,multi cell organism development,cell-cell adhesion,cell differentiation,monocyte activation et al.The further analysis indicated the expression level of some mRNAs such as COL16A1, ADAM-9,CDH2, LOX were consistent with the results of gene chip. Conclusion We can use the method of bio-informative analysis to study the data obtained from the gene chip. There are mRNAs that may play a role in the expression and function of extracellular matrix during the formation of IUA.

Key words: intrauterine adhesion, gene chip, bio-informatics

中图分类号:

R711

柳鑫, 段华, 成九梅, 陈芳, 徐倩. 重度宫腔粘连相关基因的生物信息学分析[J]. 首都医科大学学报, 2018, 39(5): 715-720.

Liu Xin, Duan Hua, Cheng Jiumei, Chen Fang, Xu Qian. Bio-informative analysis of severe intrauterine adhesion related gene[J]. Journal of Capital Medical University, 2018, 39(5): 715-720.

参考文献

[1] Cousins F L, Kirkwood P M, Saunders P T,et al.Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling[J].Sci Rep,2016, 6:36748.
[2] Clarke R, Ressom H W, Wang A, et al. The properties of high-dimensional data spaces:implications for exploring gene and protein expression data[J]. Nat Rev Cancer, 2008,8(1):37-49.
[3] Yu D,Li T C,Xia E,et al.Factors affecting reproductive outcome of hysteroscopic adhesiolysis for Asherman's syndrome[J].Fertil Steril,2008,89(3):715-722.
[4] Gan L, Duan H, Xu Q,et al. Human amniotic mesenchymal stromal cell transplantation improves endometrial regeneration in rodent models of intrauterine adhesions[J]. Cytotherapy, 2017,19(5):603-616.
[5] Gan L, Duan H, Sun F Q, et al. Efficacy of freeze-dried amnion graft following hysteroscopic adhesiolysis of severe intrauterine adhesions[J].Int J Gynaecol Obstet, 2017,137(2):116-122.
[6] Liu X, Duan H, Zhang H H. Integrated data set of microRNAs and mRNAs involved in severe intrauterine adhesion[J]. Reprod Sci, 2016,23(10):1340-1347.
[7] Maquart F X, Monboisse J C. Extracellular matrix and wound healing[J]. Pathol Biol (Paris), 2014,62(2):91-95.
[8] Alimperti S, Andreadis S T. CDH2 and CDH11 as regulators of stem cell fate decisions[J].Stem Cell Res, 2015, 14(3):270-282.
[9] Black M, Milewski D, Le T, et al. FOXF1 inhibits pulmonary fibrosis by preventing CDH2-CDH11 cadherin switch in myofibroblasts[J].Cell Rep,2018,23(2):442-458.
[10] Brasch J, Harrison O J, Ahlsen G, et al. Structure and binding mechanism of vascular endothelial cadherin:a divergent classical cadherin[J].J Mol Biol,2011,408(1):57-73.
[11] Li L,Bennett S A, Wang L. Role of E-cadherin and other cell adhesion molecules in survival and differentiation of human pluripotent stem cells[J]. Cell Adh Migr, 2012,6(1):59-70.
[12] Grässel S,Bauer R J. Collagen XVI in health and disease[J]. Matrix Biol, 2013,32(2):64-73.
[13] Li C, Nguyen H T, Zhuang Y, et al. Post-transcriptional upregulation of miR-21by type I collagen[J]. Mol Carcinog, 2011,50(7):563-570.
[14] Ratzinger S, Eble J A, Pasoldt A, et al.Collagen XVI induces formation of focal contacts on intestinal myofibroblasts isolated from the normal and inflamed intestinal tract[J]. Matrix Biol,2010,29(3):177-193.
[15] Diomede F, D'Aurora M, Gugliandolo A, et al. Biofunctionalized scaffold in bone tissue repair[J].Int J Mol Sci, 2018, 29, 19(4):E1022
[16] Gil-Cayuela C, Roselló-LLetí E, Ortega A, et al.New altered non-fibrillar collagens in human dilated cardiomyopathy:role in the remodeling process[J].PLoS One,2016, 11(12):e0168130.
[17] Roychaudhuri R, Hergrueter A H, Polverino F,et al.ADAM9 is a novel product of polymorphonuclear neutrophils:regulation of expression and contributions to extracellular matrix protein degradation during acute lung injury[J].J Immunol, 2014193(5):2469-2482.
[18] Erin N, Türker S, Elpek Ö, et al. ADAM proteases involved in inflammation are differentially altered in patients with gastritis or ulcer[J].Exp Ther Med, 2018, 15(2):1999-2005.
[19] Giebeler N, Schönefu A, Landsberg J,et al. Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis[J].Oncogene, 2017, 31,36(35):5058-5067.
[20] Mauch C, Zamek J, Abety A N, et al. Accelerated wound repair in ADAM-9 knockout animals[J]. J Invest Dermatol, 2010,130(8):2120-2130.

重度宫腔粘连相关基因的生物信息学分析

Bio-informative analysis of severe intrauterine adhesion related gene

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