GPIHBP1基因敲除小鼠在严重高三酰甘油血症急性胰腺炎导致肺损伤研究中的应用

doi:10.3969/j.issn.1006-7795.2020.04.012

首都医科大学学报 ›› 2020, Vol. 41 ›› Issue (4): 564-569.doi: 10.3969/j.issn.1006-7795.2020.04.012

GPIHBP1基因敲除小鼠在严重高三酰甘油血症急性胰腺炎导致肺损伤研究中的应用

柳鑫¹, 徐有青², 崔纯莹³, 赵志刚¹

1. 首都医科大学附属北京天坛医院药学部, 北京 100070;
2. 首都医科大学附属北京天坛医院消化内科, 北京 100070;
3. 首都医科大学药学院, 北京 100069

收稿日期:2020-03-01 出版日期:2020-08-21 发布日期:2020-07-22
通讯作者: 赵志刚 E-mail:1022zzg@sina.com
基金资助:
北京市委优秀人才项目（2018000021469G239），北京市医院管理中心青苗培育项目（QML20170507），首都医科大学科研培育基金（17JL67）。

Application of GPIHBP1 gene knockout mice in the study of lung injury induced by acute pancreatitis with severe hypertriglyceridemia

Liu Xin¹, Xu Youqing², Cui Chunying³, Zhao Zhigang¹

1. Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China;
2. Department of Gastroenterology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China;
3. College of Pharmacy, Capital Medical University, Beijing 100069, China

Received:2020-03-01 Online:2020-08-21 Published:2020-07-22
Supported by:
This study was supported by Talent Project of Beijing Municipal Committee (2018000021469G239), Beijing Municipal Administration of Hospitals' Youth Program (QML20170507), Cultivation Fund of Capital Medical University (17JL67).

摘要/Abstract

摘要： 目的旨在通过基因修饰的高三酰甘油血症小鼠建立急性胰腺炎模型，观察胰腺与肺病理改变。方法采用野生小鼠与糖基化磷脂酰肌醇锚定高密度脂蛋白结合蛋白1（glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1，GPIHBP1）基因敲除小鼠，腹腔注射雨蛙素，诱导急性胰腺炎，检测血浆脂质与不同时间下淀粉酶活性，观察胰腺与肺组织病理形态改变。结果 GPIHBP1基因敲除小鼠的血浆三酰甘油极度升高，在诱导急性胰腺炎后，淀粉酶活性明显改变，胰腺病理损伤加重，同时伴有肺损伤病变，肺泡灌洗液细胞数目明显增加。结论 GPIHBP1基因敲除小鼠诱导急性胰腺炎后，胰腺与肺病理损伤明显加重，对进一步探讨高脂血症急性胰腺炎导致肺损伤及远隔器官损伤的发病机制具有一定的意义。

关键词: 严重高三酰甘油血症, 急性胰腺炎, 肺损伤, GPIHBP1基因敲除

Abstract: Objective To establish a model of acute pancreatitis in genetically modified mice with hypertriglyceridemia and to observe pathological changes of pancreas and lung tissues. Methods Wild type and glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1(GPIHBP1) gene knockout mice with hypertriglyceridemia were used to induce acute pancreatitis via intraperitoneal injection of caerulein. Amylase activity and plasma levels of lipids were detected. Pathological changes of pancreas and lung tissues were observed. Results GPIHBP1 knockout mice showed extremely high plasma levels of triglycerides. Amylase activity was significantly altered after induction of acute pancreatitis. The pathological injury of pancreas was aggravated, accompanied by lung injury, and the cell number of alveolar lavage fluid significantly increased. Conclusion After induction of acute pancreatitis, the pathological damage of pancreas and lung tissues in GPIHBP1 knockout mice was significantly aggravated, indicating that it has certain significance for further exploring the pathogenesis of hyperlipidemic acute pancreatitis leading to lung and remote organ injury.

Key words: severe hypertriglyceridemia, acute pancreatitis, lung injury, GPIHBP1 gene knockout

中图分类号:

R657.5

柳鑫, 徐有青, 崔纯莹, 赵志刚. GPIHBP1基因敲除小鼠在严重高三酰甘油血症急性胰腺炎导致肺损伤研究中的应用[J]. 首都医科大学学报, 2020, 41(4): 564-569.

Liu Xin, Xu Youqing, Cui Chunying, Zhao Zhigang. Application of GPIHBP1 gene knockout mice in the study of lung injury induced by acute pancreatitis with severe hypertriglyceridemia[J]. Journal of Capital Medical University, 2020, 41(4): 564-569.

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GPIHBP1基因敲除小鼠在严重高三酰甘油血症急性胰腺炎导致肺损伤研究中的应用

Application of GPIHBP1 gene knockout mice in the study of lung injury induced by acute pancreatitis with severe hypertriglyceridemia

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