Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (6): 829-833.doi: 10.3969/j.issn.1006-7795.2011.06.025

• 基础研究 • Previous Articles     Next Articles

Isolation and identification of putative human epidermal stem cells

MEI Xue-ling1, LIAN Shi2   

  1. 1. Department of Dermatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;2. Department of Dermatology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
  • Received:2011-09-06 Revised:1900-01-01 Online:2011-12-21 Published:2011-12-21

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Abstract: Objective To isolate and identify keratinocytes enriched with putative human epidermal stem cells. Methods We isolated keratinocytes adherent to collagen type Ⅳ and normal primary keratinocytes. We stained cells with antibodies targeting α6-integrin and p63 under the same conditions and examined the expression of these proteins under a confocal microscope. Results Keratinocytes adherent to collagen type Ⅳ expressed high levels of p63 protein and α6-integrin but normal primary keratinocytes expressed low levels of p63 protein and α6-integrin(P<0.05). Conclusion These results indicate that keratinocytes adherent to collagen type Ⅳ are keratinocytes enriched with putative human epidermal stem cells.

Key words: epidermal stem cells, collagen type Ⅳ, α6-integrin, p63 protein

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