Journal of Capital Medical University ›› 2012, Vol. 33 ›› Issue (6): 798-802.doi: 10.3969/j.issn.1006-7795.2012.06.019

• 临床研究 • Previous Articles     Next Articles

Down-regulation of DcR3 expression by siRNA in SW480 cells

HE Ping1, WU Jixiang1, ZHONG Chenxi1, DAI Jie2, LIANG Jiexiong1, LI Yang1, XU Yingcheng1, YU Wei2   

  1. 1. Department of General Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;2. Department of Pathology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China
  • Received:2012-05-14 Revised:1900-01-01 Online:2012-12-21 Published:2012-12-21

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Abstract: Objective To investigate the inhibitory effect of small interfering RNA(siRNA) on the expression of decoy receptor 3(DcR3) in human colon carcinoma cell line SW480. Methods Two siRNA plasmid expression vectors targeting DcR3 were constructed and transfected into SW480 cells with Lipofectamine 2000. The down regulation of DcR3 expressions were detected by RT-PCR and Western blotting methods, following statistical processing. Results Compared with the control group, the sequence-specific siRNAs efficiently and specifically down-regulated the expression of DcR3 at both mRNA and protein levels. The expression inhibition of mRNA and protein of DcR3 significantly decreased(P<0.01). Conclusion The siRNA plasmid expression vector against DcR3 can inhibit the expression of DcR3 in SW480 cells efficiently and specifically. siRNA against DcR3 may be of potential value in gene therapy of cancer.

Key words: decoy receptor 3, small interfering RNA, colon carcinoma cell

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