Difference of the parotid microflora Alpha diversity of microflora between juvenile recurrent parotitis(JRP) and the normal children

doi:10.3969/j.issn.1006-7795.2018.03.026

Journal of Capital Medical University ›› 2018, Vol. 39 ›› Issue (3): 453-457.doi: 10.3969/j.issn.1006-7795.2018.03.026

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Difference of the parotid microflora Alpha diversity of microflora between juvenile recurrent parotitis(JRP) and the normal children

Li Ying¹, Qi Senrong², Hua Lin³, Shang Jiajian¹

1. Department of Pediatric, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China;
2. Department of Radiology, Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China;
3. Department of Mathematics and Bioinformatics, School of Biomedical Engineering, Capital Medical University, Beijing 100069, China

Received:2018-03-08 Online:2018-05-21 Published:2018-06-11

Abstract

Abstract: Objective To investigate the difference in the richness and diversity of the parotid microflora between juvenile recurrent parotitis(JRP) and the normal children.Methods The parotid saliva specimens were obtained from JRP and normal children(34 specimens from JRP,16 specimens from normal children) to analyze the diversity of the 16SrRNA gene sequence of of the microorganism using Illumina Hiseq 2500 PE250 high throughput sequencing platform.Results Compared with normal children, JRP had higher goods_coverage(P< 0.05), lower Chao1, abundance coverage estimator (ACE), Shannon (P < 0.05) and lower Simpson (P > 0.05).Conclusion Significant changes in the richness and diversity of parotid microflora between JRP and the normal children.

Key words: juvenile recurrent parotitis (JRP), oral microflora, diversity, richness, parotid saliva

CLC Number:

R788

Li Ying, Qi Senrong, Hua Lin, Shang Jiajian. Difference of the parotid microflora Alpha diversity of microflora between juvenile recurrent parotitis(JRP) and the normal children[J]. Journal of Capital Medical University, 2018, 39(3): 453-457.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.03.026

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I3/453

References

[1] 谢李松.儿童复发性腮腺炎病因学研究及治疗进展[J].中国实用口腔科杂志,2012,5(12):759-762.
[2] Brook I. The bacteriology of salivary gland infections[J]. Oral Maxillofac Surg Clin North Am, 2009, 21(3):269-274.
[3] Gantner S, Andersson A F, Alonso-Sáez L, et al. Novel primers for 16S rRNA-based archaeal community analyses in environmental samples[J]. J Microbiol Methods, 2011,84(1):12-18.
[4] Herlemann D P, Labrenz M, Jürgens K, et al. Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea[J].ISME J, 2011,5(10):1571-1579.
[5] Caporaso J G, Kuczynski J, Stombaugh J, et al. QⅡME allows analysis of high-throughput community sequencing data[J]. Nat Methods, 2010, 7(5):335-336.
[6] Edgar R C, Haas B J, Clemente J C, et al. UCHIME improves sensitivity and speed of chimera detection[J].Bioinformatics,2011,27(16):2194-2200.
[7] Wang Q, Garrity G M, Tiedje J M, et al. Naivebayesian classifier for rapid assignment of rrnasequences into the new bacterial taxonomy[J]. Applied and environmental microbiology, 2007,73(16):5261-5267.
[8] Chao A. Non-parametric estimation of the number of classes in a population[J]. Nat Methods,1984,11(4):265-270.
[9] Shannon C E, Weaver W. A mathematical theory of communication[J]. Bell Sys Technic J, 1948,27(4):379-423.
[10] Simpson E H. The Measurement of diversity[J]. Nature, 1949, 163(4148):688.
[11] Dewhirst F E, Chen T, Izard J, et al. The human oral microbiome[J]. J Bacteriol, 2010,192(19):5002-5017.
[12] 何金枝,徐欣,周学东.口腔微生物与全身健康研究进展[J].微生物与感染,2017,12(3):139-145.
[13] 徐欣,何金枝,周学东.口腔微生物群落在口腔与全身疾病预警中的作用[J].华西口腔医学杂志,2015,33(6):555-560.
[14] Merritt J, Kreth J, Shi W, et al. LuxS controls bacteriocin production in Streptococcus mutans through a novel regulatory component[J]. Mol Microbiol,2005,57(4):960-969.
[15] 施文元,周学东.人体口腔微生物群的相互作用[J].华西口腔医学杂,2010,28(1):1-4.
[16] Becker M R,Paster B J,Leys E J,et al. Molecular analysis of bacterial species associated with childhood caries[J].J Clin Microbiol,2002,40(3):1001-1009.
[17] 李志艳,赵翀,刘静,等.青海汉族和藏族高龋患者口腔微生物多样性差异研究[J].牙体牙髓牙周病学杂志,2017,27(5):250-253,265.
[18] Papadopoulou-Alataki E, Chatziavramidis A, Vampertzi O, et al. Evaluation and management of juvenile recurrent parotitis in children from northern Greece[J]. Hippokratia, 2015, 19(4):356-359.
[19] 李琰.中药长皮膏对慢性下肢静脉性溃疡的微生态调控作用的研究[D].上海:上海中医药大学,2015:1-140.
[20] Roby B B, Mattingly J, Jensen E L, et al. Treatment of juvenile recurrent parotitis of childhood:an analysis of effectiveness[J]. JAMA Otolaryngol Head Neck Surg, 2015, 141(2):126-129.
[21] 王少果,游祥磊,车春晓,等.16SrRNA高通量测序研究有龋者唾液微生物群落结构及多样性[J].口腔医学研究,2015,31(5):461-465.
[22] 陈慧.口腔微生物组的结构分析及与疾病的关系研究[D].上海:上海交通大学,2016:1-189.
[23] 许志亮. 非手术根管再治疗-根尖手术联合治疗难治性根尖周炎[J].首都医科大学学报,2015,36(3):478-482.

Difference of the parotid microflora Alpha diversity of microflora between juvenile recurrent parotitis(JRP) and the normal children

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