Abstract: Objective To investigate the stereoselectivity recognition of β-cyclodextrin or 2,3,6-tri-o-methyl-β-cyclodextrin as the chiral selector with phencynonate, a novel chiral anticholinergic agent. Methods β-Cyclodextrin or 2, 3, 6-tri-o-methyl-β-cyclodextrin was mixed with phencynonate in equal volume and formed the complexes, respectively. The samples were injected directly to the tandem quadrupole mass spectrometry. In positive ion mode, the relative abundances and the combination rate of β-cyclodextrin or 2, 3, 6-tri-o-methyl-β-cyclodextrin with phencynonate isomer complex were analyzed. Results 2,3,6-Tri-o-methyl-β-cyclodextrin had strong recognition ability of stereoselectivity to chiral phencynonate isomers, but the chiral recognition ability of β-cyclodextrin to phencynonate isomers was not significant.Conclusion There is markedly stereoselective interaction between 2,3,6-tri-o-methyl-β-cyclodextrin and phencynonate.

Key words: β-cyclodextrin, 6-tri-o-methyl-β-cyclodextrin, chiral recognition, phencynonate, tandem quadrupole mass spectrometry