Study on micronucleus frequency in human lymphocyte exposed to occupational lead

doi:10.3969/j.issn.1006-7795.2013.05.017

Abstract

Abstract:

Objective To explore the genetic toxicity of occupational lead exposure. Methods Sixty workers who came to our institute for occupational health examination from July to September 2012 were divided into two groups: lead group 38 people and non-lead group 22 people, all males. The uric lead was measured by graphite furnace atomic absorption spectrometry (GF-AAS), the Zn-protoporphyrin (ZPP) in blood was measured by surface fluorescence method, and lymphocyte micronucleus frequency (MNF, ‰) was detected using micro-whole blood culture method. Then statistical analysis was applied. Results There was no statistical difference in urine lead between lead group and non-lead group, but the differences in ZPP and MNF were significant (P<0.05). Furthermore, the result of statistical analysis showed that there was a strong positive correlation(r_s=0.778) between ZPP and MNF in lead exposed workers. Conclusion Occupational lead could increase the MNF in lead exposed workers, inducing chromosome aberration.

Key words: lead, micronucleus frequency, Zn-protoporphyrin, genotoxicity

CLC Number:

R135.1⁺1

LIANG Jing, LUO Huan, CHEN Bin, CHANG Xiaoyan, WANG Ping, YU Guixin, LUO Hu . Study on micronucleus frequency in human lymphocyte exposed to occupational lead[J]. Journal of Capital Medical University, 2013, 34(5): 720-723.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.05.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I5/720

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