Comparsion of structure of protein N-glycosylation profiles between age groups

doi:10.3969/j.issn.1006-7795.2014.06.019

Abstract

Abstract:

Objective To explore difference of plasma N-glycosylation structure between age groups. Methods Based on cluster sampling, 212 residents of a certain community were selected and the serum N-glycans profiles were obtained by high performance liquid chromatography(HPLC).Two independent samples t test or nonparametric tests were used to compare the N-glycosylation structure between age groups divided by 35. Results The average age of the 212 subjects was(37.78±17.89) years, and the sex ratio was 0.88. More digalactosylated glycans, monosialylated glycans, core-fucosylated glycans and biantennary glycans were detected in 35 years or more, whereas more biantennary non-galactosylated glycans, disialylated biantennary glycans, trisialylated glycans and triantennary glycans were seen in younger than 35 years(P≤0.03). Conclusion The N-glycome profile in serum is age dependent. This should be taken into consideration in the development of serum glycome markers.

Key words: N-glycosylation, profiles, HPLC

CLC Number:

R195

Liu Xiangtong, Wang Youxin, Yang Xinghua, Dong Jing, Wang Wei, Guo Xiuhua. Comparsion of structure of protein N-glycosylation profiles between age groups[J]. Journal of Capital Medical University, 2014, 35(6): 779-784.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.06.019

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I6/779

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