Effects of botulinum toxin A combined with low dose gabapentin on the NK-1 receptor internalization at spinal cord dorsal horn in rats with incisional pain

doi:10.3969/j.issn.1006-7795.2017.03.001

Abstract

Abstract: Objective To evaluate the effects of botulinum toxin A combined with low dose gabapentin on the neurokinin-1 (NK-1) receptor internalization at spinal cord dorsal horn in rats with incisional pain. Methods Male Sprague-Dawley rats, weighing 280-300 g, aged 6-8 weeks, were used in the study. Rats were randomly selected and divided into 5 groups (n=9 each) using a random number table:control group (Control group), incisional pain group (Saline group), gabapentin group (GBP group), botulinum toxin A group (BoNT/A group), botulinum toxin A combined with gabapentin group (G+B group). At 24 h before operation, botulinum toxin A 0.5U (in 10 mL of normal saline) was injected intrathecally in BoNT/A group and G+B group. At 30 min before operation, gabapentin 50 mg was injected intrathecally in GBP group and G+B group. At 3 h after operation, 6 rats in each group were selected to measure the cumulative pain scores (CPS) and mechanical paw withdrawal threshold (PWT) in the right hindpaw; besides, 3 rats in each group were selected and sacrificed, and the lumbar segment (L4,5) of the spinal cord was removed for determination of the expression of NK-1 receptors in the spinal dorsal horn by immunofluorescence. Results Compared with Control group, the CPS was significantly increased, the PWT was significantly decreased, and the expression of NK-1 receptors in the spinal dorsal horn was significantly up-regulated in Saline group, GBP group, BoNT/A group and G+B group at 3 h after operation(P<0.05). Compared with Saline group, the CPS was significantly decreased, the PWT was significantly increased, and the expression of NK-1 receptors in the spinal dorsal horn was significantly down-regulated in BoNT/A group and G+B group at 3 h after operation (P<0.05), and no significant change was found in group GBP (P>0.05). Compared with BoNT/A group, the CPS was significantly decreased, the PWT was significantly increased, and the expression of NK-1 receptors in the spinal dorsal horn was significantly down-regulated in G+B group at 3 h after operation (P<0.05). Conclusion Low doses of gabapentin alone may have no effect on postoperative pain, but when it is coadministrated with BoNT/A, it can greatly enhance the analgesic effect of BoNT/A. The analgesic mechanism may be due to inhibition of the internalization of NK-1 receptor at spinal cord horn in a rat model of incisional pain.

Key words: botulinum toxin type A, gabapentin, pain, neurokinin-1 receptors, spinal cord

CLC Number:

R971

Li Xueyang, Wang Yun, Sun Yuqing, Ma Danxu, Wu Anshi, Yue Yun. Effects of botulinum toxin A combined with low dose gabapentin on the NK-1 receptor internalization at spinal cord dorsal horn in rats with incisional pain[J]. Journal of Capital Medical University, 2017, 38(3): 331-335.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.03.001

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I3/331

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