Bufalin induces necroptosis in human gastric cancer SGC-7901 cells

doi:10.3969/j.issn.1006-7795.2019.02.018

Journal of Capital Medical University ›› 2019, Vol. 40 ›› Issue (2): 251-256.doi: 10.3969/j.issn.1006-7795.2019.02.018

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Bufalin induces necroptosis in human gastric cancer SGC-7901 cells

Gong Pengchao¹, Li Yanlan¹, Kong Cuicui², Tian Xin¹

1. Molecular Oncology Laboratory of Cancer Research Institute, The First Affiliated Hospital of China Medical University, Shenyang 110001, China;
2. Department of Central Laboratory, The Cancer Hospital of China Medical University, Shenyang 110001, China

Received:2018-05-22 Online:2019-03-21 Published:2019-04-15
Supported by:
This study was supported by National Natural Science Foundation of China(8157140503).

Abstract

Abstract: Objective To investigate the necroptosis mechanism induced by Bufalin in human gastric cancer SGC-7901 cells. Methods SGC-7901 cells were cultured in vitro. The cells in the control group were cultured in RPMI 1640 medium, while those in the experimental group were exposed to various concentrations of Bufalin(50,100,150,200 nmol/L). After treatment for 48 h, the cell survival rate was assessed by MTT assay. Cell ultrastructures were observed by transmission electron microscope. Morphological changes in the nuclei were observed by DAPI fluorescence staining. Cellular necroptosis was assayed by flow cytometry. Western blotting was used to analyze the expression of receptor interacting protein kinase 1 (RIP1) in the necroptosis pathway. Results MTT assay showed that Bufalin had a significant effect on the growth of SGC-7901 cells(P<0.05). Transmission electron microscopy can be used to observe characteristic changes in cell necrosis, such as disruption of cell membranes, production of intracellular vacuoles, and disintegration of organelles. DAPI staining showed that after treatment with 100 nmol/L for 48 h, there were no obvious changes in cell apoptosis, such as nuclear condensation, nuclear fragmentation and formation of apoptotic bodies. The cell necrosis rate was higher than control group(P<0.05). Bufalin promotes the expression of RIP1, a key protein of necroptosis pathway(P<0.05). Conclusion Bufalin efficiently induced cell death through necroptosis pathway in SGC-7901 cells.

Key words: Bufalin, human gastric cancer cell, necroptosis

CLC Number:

R735.2

Gong Pengchao, Li Yanlan, Kong Cuicui, Tian Xin. Bufalin induces necroptosis in human gastric cancer SGC-7901 cells[J]. Journal of Capital Medical University, 2019, 40(2): 251-256.

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Bufalin induces necroptosis in human gastric cancer SGC-7901 cells

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