Diagnosis value of plasma miRNA-320C and miRNA-6088 in diagnosis of type Ⅱ diabetic nephropathy

doi:10.3969/j.issn.1006-7795.2019.03.026

Abstract

Abstract: Objective To study the diagnosis value of plasma miRNA-320C (miR-320C) and miRNA-6088 (miR-6088) in type Ⅱ diabetic nephropathy (T2D). Methods Fifty-six T2D patients for T2D group, 105 type Ⅱ diabetic nephropathy (DN) for DN group and 52 healthy people for normal control (NC) group were recruited from Jun. 2015 to Dec. 2016 in Beijing Anzhen Hospital, Capital Medicical University. The expression of circulating miR-320C andmiR-6088 were detected by RT-qPCR. Pearson analysis was used to analyze the correlation between miR-320C or miR-6088 and clinical data. And receiver operating characteristic(ROC) curve was drawn to test the diagnostic value of miR-320C and miR-6088.Results The expression of circulating miR-320C and miR-6088 in DN group was higher than NC group (P<0.05) and T2D group (P<0.05). Pearson correlation analysis showed that miR-320C or miR-6088 was positively correlated with urinary albumin excretion rate (UAER), negatively correlated with estimated glomerular filtration rate (eGFR), while had no correlation with the plasma levels of human glycated hemoglobin A1C (HbA1C),triglycerides, low density lipoprotein-cholesterol (LDL-C) and diabetes duration, body mass index. ROC curves of miR-320C and miR-6088 presented an area under curve of 0.834 and 0.753, respectively. After treatment, both miR-320C and miR-6088 expression were significantly decreased (P<0.05).Conclusion Circulating miR-320C and miR-6088 were highly expressed in type Ⅱ diabetic nephropathy, which may serve as useful diagnostic and prognostic biomarkers with type Ⅱ diabetic nephropathy.

Key words: miR-320C, miR-6088, type Ⅱ diabetic nephropathy, biomarker

CLC Number:

R446

Wang Rui, Wu Suping, Xu Pinying. Diagnosis value of plasma miRNA-320C and miRNA-6088 in diagnosis of type Ⅱ diabetic nephropathy[J]. Journal of Capital Medical University, 2019, 40(3): 466-472.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.03.026

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I3/466

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