Observation on the improvement of Si-Miao-Yong-An decoction based on KAS-seq sequencing and its promotion of wound healing in mice with diabetic foot

doi:10.3969/j.issn.1006-7795.2024.06.015

Abstract

Abstract: Objective Based on modern scientific technology, the Si-Miao-Yong-An decoction was improved to verify its effect in promoting the healing of diabetic foot wounds. Methods The kethoxal-assisted single stranded DNA sequencing (KAS-seq) technology was utilized to detect wound tissues from diabetic foot patients and normal wound tissues, to screen for key genes associated with diabetic foot. Combining network pharmacology, targeted traditional Chinese medicines were identified based on these key genes to modify the traditional formula Si-Miao-Yong-An decoction. Finally, the therapeutic effect of the modified formula was verified through an animal model of diabetic foot. Results Through clinical sample sequencing analysis, 8 key genes, including CREB1, GRID2, PIK3R3, DRD2, ADORA1, TNFSF11, EDNRB, HTR1B were obtained. These genes were targeted to Huang Bai, Cang Zhu, Di Huang, and Ban Xia, four traditional Chinese medicines combined in the prescription. On the 7th day of intragastric intervention, the wound healing efficiency of the S+ group mice was significantly higher than that of the control group (P<0.05), and on the 12th day, the wound healing rate of the S+ group mice was significantly higher than that of the S group (P<0.05). The target points of the modified prescription were more enriched in the neural system, hypoxia, and blood circulation regulation-related pathways compared to the original prescription. Conclusions Compared to the original prescription, the modified prescription has a better effect on promoting the healing of diabetic foot wounds. The related mechanism may be associated with the regulation of inflammation, improvement of peripheral nerve damage, and promotion of wound healing pathways. This approach also provides a new perspective and a basis in modern scientific theory for the clinical application and modification of traditional Chinese medicine prescriptions.

Key words: modified si-miao-yong-an decoction, diabetic foot ulcer, Chinese herbal compound, KAS-seq, network pharmacology

CLC Number:

R932

Li Xuehui, Chen Hangyu, Ma Ruiyao, Lin Jian, Li Linlin. Observation on the improvement of Si-Miao-Yong-An decoction based on KAS-seq sequencing and its promotion of wound healing in mice with diabetic foot[J]. Journal of Capital Medical University, 2024, 45(6): 1050-1061.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2024.06.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2024/V45/I6/1050

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