关键词: 免疫抑制, 凉血解毒方, T淋巴细胞活化, 银屑病, 血热证

Abstract: Objective To study the effects of Liang Xue Jie Du Fang(LXJDF) on proliferation, activation and cytokine production of Jurkat T lymphocytes, to elucidate the mechanism of Chinese medicine for blood heat in psoriasis, and to provide the evidence for clinical treatment. Methods Jurkat E6-1 T lymphocytes were stimulated with PDB after pre-treatment with different dose LXJDF pastille serum for 3 h. The CCK-8 assay was used to detect T cell proliferation; flow cytometry was adopted to observe CD69 expression, an early marker of T cell activation; and the level of TNF-α was measured by enzyme linked immune sorbent assay(ELISA). Results LXJDF pastille serum can obviously reduce the T lymphocyte's quantity, except for drug toxicity. All kinds of LXJDF pastille serum could down-regulate CD69 expression. And TNF-α production could be inhibited by different doses of pastille serum. Conclusion LXJDF can significantly inhibit PDB mediated T lymphocyte's proliferation, activation and cytokine production.

Key words: immunosuppression, Liang Xue Jie Du Fang, T lymphocytes activation, psoriasis, blood heat