首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (6): 785-789.doi: 10.3969/j.issn.1006-7795.2013.06.001

• 过敏性鼻炎和慢性鼻窦炎 • 上一篇    下一篇

重塑相关因子在慢性鼻窦炎的表达

伯铭羽1, 王向东1,2, 王鸿2, 宋贝贝1, 李颖2, 范尔钟2, 张罗1,2   

  1. 1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科, 北京 100050;
    2. 北京市耳鼻咽喉科研究所, 耳鼻咽喉头颈科学教育部重点实验室, 鼻病研究北京市重点实验室, 北京 100005
  • 收稿日期:2013-10-08 出版日期:2013-12-21 发布日期:2013-12-13
  • 通讯作者: 张罗 E-mail:dr.luozhang@gmail.com
  • 基金资助:

    国家杰出青年科学基金（81025007）；北京市自然科学基金重点项目（7131006）；北京市卫生系统高层次卫生技术人才培养计划（2011-3-039）；鼻病研究北京市重点实验室2012年阶梯计划项目（Z121107009212032）；北京市人力资源和社会保障局留学归国人员择优资助项目。

Expression of remodeling associated factors in the tissue of chronic rhinosinusitis

BO Mingyu1, WANG Xiangdong1,2, WANG Hong2, SONG Beibei1, LI Ying2, FAN Erzhong2, ZHANG Luo1,2   

  1. 1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100050, China;
    2. Beijing Institute of Otorhinolaryngology, Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Key Laboratory of Nasal Diseases, Beijing 100005, China
  • Received:2013-10-08 Online:2013-12-21 Published:2013-12-13
  • Supported by:

    This study was supported by the National Science Foundation for Distinguished Young Scholar(81025007);Beijing Municipal Natural Science Foundation Key Program(7131006);High-level Talent Project of Beijing Health Bureau(2011-3-039);Beijing Key Laboratory of Nasal Diseases(Z121107009212032);Returnee Founding of Beijing Human Resources and Social Security Bureau.

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246

被引次数

15

摘要：

目的 研究重塑相关因子基质金属蛋白酶（matrix metalloproteinases，MMPs）、组织金属蛋白酶抑制物（tissue inhibitor of metalloproteinase，TIMPs）以及转化生长因子β1（transforming growth factor β1，TGF-β1）和白蛋白（albumin）在慢性鼻窦炎（chronic rhinosinusitis，CRS）组织中的表达。方法 按数字表法随机选择95例鼻息肉组织（chronic rhinosinusitis with nasal polyps，CRSwNP），33例慢性鼻窦炎组织（chronic rhinosinusitis without nasal polyps，CRSsNP）和24例对照鼻甲黏膜。采用组织匀浆，酶联免疫吸附测定（enzyme linked immunosorbent assay，ELISA）检测MMP-1，2，3，7，8，9，12，13以及TGF-β1和白蛋白。结果 MMP7，8，9在CRSwNP中的表达高于对照组，并且中性粒细胞型鼻息肉（neutrophilic CRSwNP，Neu CRSwNP）组织中MMP-8明显高于嗜酸性粒细胞型鼻息肉（eosinophilic CRSwNP，Eos CRSwNP）组织。TIMP-1在CRSsNP鼻窦黏膜组织中的表达明显高于对照组和鼻息肉组织。TIMP-2在鼻息肉组织和CRSsNP鼻窦黏膜组织中的表达明显高于对照组。TGF-β1的表达在各组间没有差异，而鼻息肉组织中albumin的表达明显高于CRSsNP鼻窦黏膜和对照组黏膜。结论 MMPs和TIMPs在不同炎性反应类型的CRS组织重塑中可能发挥重要的作用。

关键词: 不伴息肉的慢性鼻窦炎, 伴息肉的慢性鼻窦炎, 基质金属蛋白酶, 组织金属蛋白酶抑制物

Abstract:

Objective To evaluate the expression and roles of matrix metalloproteinases(MMPs), tissue inhibitor of metalloproteinase(TIMPs), transforming growth factor β1(TGF-β1) and albumin in chronic rhinosinusitis without nasal polyps(CRSsNP) and with nasal polyps(CRSwNP) in northern China. Methods CRSwNP tissue specimens of 95 cases, sinus mucosa of 33 CRSsNP cases and 24 cases of control were collected and analyzed in the present study. ELISA was used to measure MMP-1, 2, 3, 7, 8, 9, 12, 13, TIMP-1, 2, 3, 4, TGF-β1 and albumin in homogenization of nasal mucosa and polyp tissue. Results MMP-7, 8 and 9 in CRSwNP tissue were significantly higher than those in control tissue. MMP-8 in nasal polyp tissue of neutrophilic CRSwNP(Neu CRSwNP) was significantly higher than that of eosinophilic CRSwNP(Eos CRSwNP). TIMP-1 in CRSsNP tissue was significantly higher than that in CRSwNP and control tissue, TIMP-2 in CRSsNP and CRSwNP tissue was significantly higher than that in control tissue. There were no significant difference in TGF-β1 among the three groups. But albumin in CRSwNP tissue was significantly higher than that in CRSwNP and control tissue. Conclusion Different MMPs and TIMPs may play different roles in the remodeling of CRSsNP and CRSwNP.

Key words: chronic rhinosinusitis without nasal polyps, chronic rhinosinusitis with nasal polyps, matrix metalloproteinases, tissue inhibitor of metalloproteinase

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