首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (1): 81-86.doi: 10.3969/j.issn.1006-7795.2014.01.018

• 艾滋病基础与临床研究 • 上一篇    下一篇

合并中枢神经系统机会性感染患者的中枢HIV-1载量和变异性增高

刘远志1, 陈兵刚2, 丁渭3, 乔录新3, 赵青霞4, 吴昊1, 陈德喜3, 张玉林1   

  1. 1. 首都医科大学附属北京佑安医院感染中心, 北京 100069;
    2. 山东省滨州市中心医院感染内科, 山东滨州 251700;
    3. 北京市肝病研究所, 北京 100069;
    4. 河南省传染病医院感染科, 郑州 450015
  • 收稿日期:2013-12-03 出版日期:2014-02-21 发布日期:2014-02-21
  • 通讯作者: 张玉林 E-mail:13051222056@126.com
  • 基金资助:

    国家自然科学基金(81371399,81272266,30910103915);“十二五”国家科技支撑计划重大项目(2012ZX10001-002,2012ZX10001-003,2012ZX10001-004);北京市艾滋病研究重点实验室(BZ0089)。

High levels of divergent HIV-1 quasispecies in neurological opportunistic infections in China

Liu Yuanzhi1, Chen Binggang2, Ding Wei3, Qiao Luxin3, Zhao Qingxia4, Wu Hao1, Chen Dexi3, Zhang Yulin1   

  1. 1. Department of Infectious Diseases, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China;
    2. Department of Infectious Diseases, Binzhou Central Hospital, Binzhou 251700, Shandong Province, China;
    3. Beijing Institute of Hepatology, Beijing 100069, China;
    4. Department of Infectious Diseases, Infectious Diseases Hospital of Henan Province, Zhengzhou 450015, China
  • Received:2013-12-03 Online:2014-02-21 Published:2014-02-21
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (81371399, 81272266, 30910103915);Key Projects of the National infectious diseases special research fund during the Twelfth Five-year Research Program of China (2012ZX10001-002, 2012ZX10001-003, 2012ZX10001-004);Beijing Key Laboratory of AIDS (BZ0089).

摘要:

目的 探讨合并中枢神经系统机会性感染患者的中枢人类免疫缺陷病毒-1(human immunodeficiency virus-1,HIV-1)病毒学特征。方法 40例输血感染HIV-1且处于艾滋病期人群,其中24例合并中枢神经系统机会性感染者(实验组),16例不合并中枢神经系统机会性感染者(对照组)。所有患者都进行外周血和脑脊液(cerebro-spinal fluid,CSF)病毒载量检测,并通过多重反转录聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)法对中枢HIV-1包膜蛋白C2-V5区进行扩增测序和序列分析。结果 两组患者间血浆HIV-1病毒载量差异无统计学意义,但实验组脑脊液HIV-1病毒载量明显增高;两组患者间CD4小计数差异无统计学意义;实验组和对照组血浆与脑脊液病毒载量,以及脑脊液病毒载量与CD4计数均无明显线性相关性;针对中枢HIV-1包膜蛋白C2-V5区序列分析显示,实验组中枢HIV-1病毒变异性明显增加。结论 合并中枢神经系统机会性感染患者的中枢HIV-1载量和病毒变异性增高。

关键词: 艾滋病, 中枢神经系统, HIV-1, 机会性感染, 病毒载量, 变异性

Abstract:

Objective To investigate virological characteristics of central nervous system (CNS) HIV-1 in AIDS patients with CNS opportunistic infection (OIs).Methods Totally 24 cases with CNS OIs and 16 non-CNS-OIs (control) cases were selected from AIDS patients who were infected with HIV-1 by paid blood donor in Henan province. HIV-1 loads of plasma and cerebrospinal fluid (CSF) were detected by reverse transcriptase polymerase chain reaction, and C2-V5 area of HIV-1 envelope region was amplified from viral quasispecies isolated from CSF by nest polymerase chain reaction. Results CSF HIV-1 load was significantly higher in cases with CNS OIs, but no high plasma HIV-1 load was found in these patients. CD4 counts were not significantly different between two groups. And, no straight linear correlations were found between CSF viral load and plasma viral load, CSF viral load and CD4 counts in either group. HIV-1 quasispecies isolated from the CSF of CNS OIs patients had a high diversity as the nucleotide sequence analysis of C2-V5 region.Conclusion These results suggest that high levels of divergent HIV-1 quasispecies in the CNS which probably contribute to opportunistic infections.

Key words: acquired immuno deficiency syndrome(AIDS), central nervous system, HIV-1, opportunistic infections, viral load, divergence

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