ether-a-go-go相关基因通道在胰岛β-细胞中的作用

doi:10.3969/j.issn.1006-7795.2019.01.008

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (1): 40-44.doi: 10.3969/j.issn.1006-7795.2019.01.008

• 糖尿病研究从基础到临床 • 上一篇下一篇

ether-a-go-go相关基因通道在胰岛β-细胞中的作用

赵苗妙^1,2,3, 元沙沙^2,3,4, 李奇^1,2,3, 卢晶^1,2,3, 黄海霞^5,6, 杨金奎^1,2,3

1. 首都医科大学附属北京同仁医院内分泌科, 北京 100730;
2. 糖尿病防治研究北京市重点实验室, 北京 100730;
3. 北京市糖尿病研究所, 北京 100730;
4. 首都医科大学附属北京潞河医院内分泌科, 北京 101149;
5. 首都医科大学基础医学院生理与病理生理学系, 北京 100069;
6. 首都医科大学代谢紊乱相关心血管疾病北京市重点实验室, 北京 100069

收稿日期:2018-11-08 出版日期:2019-01-21 发布日期:2019-01-23
通讯作者: 杨金奎 E-mail:jinkui.yang@gmail.com
基金资助:
国家自然科学基金（81370946，81400824，81800688），北京市自然科学基金（7131005），首都医科大学附属北京同仁医院科研基金（TRYY-KYJJ-2016-013）。

Function of ether-a-go-go related gene channel in pancreatic β-cells

Zhao Miaomiao^1,2,3, Yuan Shasha^2,3,4, Li Qi^1,2,3, Lu Jing^1,2,3, Huang Haixia^5,6, Yang Jinkui^1,2,3

1. Department of Endocrine and Metabolic Diseases, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
2. Beijing Key Laboratory of Diabetes Research and Care, Beijing 100730, China;
3. Beijing Diabetes Institute, Beijing 100730, China;
4. Department of Endocrine and Metabolic Diseases, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, China;
5. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
6. Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, Capital Medical University, Beijing 100069, China

Received:2018-11-08 Online:2019-01-21 Published:2019-01-23
Supported by:
This study was supported by National Natural Science Foundation of China (81370946,81400824,81800688), Natural Science Foundation of Beijing (7131005) and Foundation of Beijing Tongren Hospital, Capital Medical University (TRYY-KYJJ-2016-013).

摘要/Abstract

摘要： 目的探究ether-a-go-go相关基因（ether-a-go-go related gene，ERG）通道在胰岛β-细胞中的作用。方法提取野生型（wild type，WT）与ERG基因敲除型（knockout，KO）小鼠胰岛β-细胞，利用全细胞膜片钳技术记录钾离子电流与动作电位，分析敲除ERG基因后钾电流与动作电位的变化。对两组小鼠进行腹腔注射葡萄糖耐量实验，分析基因敲除后小鼠糖耐量的变化，探究ERG基因是否对体内血糖浓度有影响。结果在两组小鼠胰岛β-细胞中记录到的钾离子电流均呈现内向整流趋势，KO组钾离子电流明显减小，动作电位时程明显延长。动物实验中，KO组小鼠糖刺激2 h后血糖浓度较WT组显著降低。结论 ERG通道电流是胰岛β-细胞中钾离子电流的重要组成部分，可显著影响动作电位时程，进而影响体内血糖浓度。

关键词: ether-a-go-go相关基因, 胰岛β-细胞, 膜片钳, 血糖

Abstract: Objective To investigate the function of ether-a-go-go related gene (ERG) channel in pancreatic β-cells. Methods Whole-cell patch clamp technique was conducted to record the potassium currents and action potential in wild type (WT) and ERG knockout (KO) primary cultured pancreatic β-cells. Intra-peritoneal glucose tolerant test (IPGTT) was performed to measure the blood glucose levels in both WT and KO mice. Results KO pancreatic β-cells had decreased potassium currents and prolonged action potential duration 90% compared with WT ones. KO mice had better blood glucose levels during IPGTT. Conclusion ERG plays a role in the potassium currents and action potential in pancreatic β-cells, which may affect the blood glucose levels in vivo.

Key words: ether-a-go-go related gene, pancreatic β-cell, patch-clamp, blood glucose

中图分类号:

赵苗妙, 元沙沙, 李奇, 卢晶, 黄海霞, 杨金奎. ether-a-go-go相关基因通道在胰岛β-细胞中的作用[J]. 首都医科大学学报, 2019, 40(1): 40-44.

Zhao Miaomiao, Yuan Shasha, Li Qi, Lu Jing, Huang Haixia, Yang Jinkui. Function of ether-a-go-go related gene channel in pancreatic β-cells[J]. Journal of Capital Medical University, 2019, 40(1): 40-44.

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ether-a-go-go相关基因通道在胰岛β-细胞中的作用

Function of ether-a-go-go related gene channel in pancreatic β-cells

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