山茱萸环烯醚萜苷对创伤性脑损伤大鼠认知功能及tau蛋白磷酸化的影响

doi:10.3969/j.issn.1006-7795.2020.03.015

首都医科大学学报 ›› 2020, Vol. 41 ›› Issue (3): 397-402.doi: 10.3969/j.issn.1006-7795.2020.03.015

• 中药有效成分治疗脑损伤 • 上一篇下一篇

山茱萸环烯醚萜苷对创伤性脑损伤大鼠认知功能及tau蛋白磷酸化的影响

马登磊¹, 张旭², 张丽¹, 李雅莉¹, 张兰¹, 李林¹

1. 首都医科大学宣武医院药学部神经变性病教育部重点实验室北京市神经药物工程研究中心, 北京 100053;
2. 首都医科大学宣武医院中心实验室, 北京 100053

收稿日期:2020-02-29 出版日期:2020-06-21 发布日期:2020-06-17
通讯作者: 李林 E-mail:linlixw@126.com
基金资助:
国家自然科学基金（81874351，81673406），北京市高层次卫生技术人才计划（2011-1-7，2014-2-014），北京市首都科技领军人才培养工程（Z191100006119017），北京市医院管理中心"登峰"计划专项（DFL20190803）。

Effects of cornel iridoid glycoside on cognitive function and tau phosphorylation in rats with traumatic brain injury

Ma Denglei¹, Zhang Xu², Zhang Li¹, Li Yali¹, Zhang Lan¹, Li Lin¹

1. Department of Pharmacy, Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing Engineering Research Center for Nerve System Drugs, Beijing 100053, China;
2. Central Laboratory, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Received:2020-02-29 Online:2020-06-21 Published:2020-06-17
Supported by:
This study was supported by National Natural Science Foundation of China (81874351, 81673406), Beijing High-level Health and Technical Personal Plan (2011-1-7, 2014-2-014), Capital Science and Technology Leading Talent Training Project (Z191100006119017), Beijing Hospitals Authority Ascent Plan (DFL20190803).

摘要/Abstract

摘要： 目的观察山茱萸环烯醚萜苷（cornel iridoid glycoside，CIG）对创伤性脑损伤（traumatic brain injury，TBI）大鼠认知功能及tau蛋白过度磷酸化的影响。方法采用改良自由落体Feeney法致右侧大脑皮质损伤制备TBI大鼠模型。造模后3 h开始给药，持续给药28 d。应用避暗试验检测大鼠被动回避记忆能力，应用Fluoro Jade B染色检测变性神经元，采用Western blotting法检测tau蛋白磷酸化的水平。结果 TBI造模术后28 d，模型组大鼠在避暗试验中的错误次数增多，海马区变性神经元增多，创伤部位tau蛋白磷酸化水平增高。CIG灌胃给药28 d能够改善TBI大鼠的被动回避记忆功能障碍，减轻海马区神经元退行性病变，抑制TBI大鼠脑创伤部位tau蛋白在Thr205和Ser396位点的过度磷酸化。结论 CIG对脑创伤所致认知功能障碍可能具有治疗作用。

关键词: 山茱萸环烯醚萜苷, 创伤性脑损伤, 认知障碍, tau蛋白过度磷酸化, 神经元变性

Abstract: Objective To investigate the effects of cornel iridoid glycoside (CIG) on cognitive impairment and tau hyperphosphorylation induced by traumatic brain injury (TBI) in rats. Methods TBI model was induced with the modified Feeney's method on the right cerebral cortex in male adult rats. Drug administration started at 3 h after TBI surgery and lasted for 28 days. The passive avoidance memory function was measured by step through test. Fluoro Jade B staining was used to detect neurodegeneration in the brain. The expression of tau phosphorylation was determined by Western blotting. Results At the 28th day after TBI surgery, the model group rats showed an increase in the error times in the step through test, the number of degenerated neurons in the hippocampus, and the expression of phosphorylated tau protein in the wound site. Intragastric administration of CIG for 28 days ameliorated the passive avoidance memory impairment, alleviated neurodegenerative lesions in the hippocampus, and decreased the hyperphosphorylation of tau protein at Thr205 and Ser309 sites near the injury site in TBI rats. Conclusion The results suggest that CIG may be beneficial to treating the cognitive impairment induced by brain trauma.

Key words: cornel iridoid glycoside, traumatic brain injury, cognitive impairment, tau hyperphosphorylation, neurodegeneration

中图分类号:

R743

马登磊, 张旭, 张丽, 李雅莉, 张兰, 李林. 山茱萸环烯醚萜苷对创伤性脑损伤大鼠认知功能及tau蛋白磷酸化的影响[J]. 首都医科大学学报, 2020, 41(3): 397-402.

Ma Denglei, Zhang Xu, Zhang Li, Li Yali, Zhang Lan, Li Lin. Effects of cornel iridoid glycoside on cognitive function and tau phosphorylation in rats with traumatic brain injury[J]. Journal of Capital Medical University, 2020, 41(3): 397-402.

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山茱萸环烯醚萜苷对创伤性脑损伤大鼠认知功能及tau蛋白磷酸化的影响

Effects of cornel iridoid glycoside on cognitive function and tau phosphorylation in rats with traumatic brain injury

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