豚鼠内耳前庭感觉毛细胞的分离

首都医科大学学报 ›› 1994, Vol. 15 ›› Issue (2): 112-114.

豚鼠内耳前庭感觉毛细胞的分离

刘芝源, 宋本波, 王淑春

北京市耳鼻咽喉科研究所

收稿日期:1993-03-27 修回日期:1900-01-01 出版日期:1994-04-15 发布日期:1994-04-15

Isolation and Identification of Inner Ear Vestibular Hair Cells of Guinea Pig

Liu Zhiyuan, Song Benbo, Wang Shuchun

Beijing Institute of Otorhinolaryngology

Received:1993-03-27 Revised:1900-01-01 Online:1994-04-15 Published:1994-04-15

摘要/Abstract

摘要： 用机械分离法从豚鼠前庭感觉上皮中分离出不同种类的前庭感觉毛细胞(VHC).VHC主要可分为Ⅰ型和Ⅱ型细胞.两种细胞形态虽有所差异,但一般均有四形或瓶形胞体,胞核位于细胞基部,胞颈和纤毛变化较大.细胞的活性可通过1%中性红染色鉴定,发现在室温下,1h内大约90%以上的分离VHC可维持其正常形态和活性.并参照对外毛细胞形态的研究提出活性VHC的鉴别标准.此项工作为进一步研究VHC的感觉机制和某些疾病的发生机理打下了基础.

关键词: 内耳, 前庭感觉毛细胞(VHC), 存活时间, 豚鼠

Abstract: Living vestibular hair cells(VHCs)were separated from vestibular sensory epithelium of guinea pig by micromanipulation.TypeⅠand typeⅡcells could be identified. Although their form varied :cell body of variable roundness,and neck and cilia of different lengths,they have a round basal portion containing the nucleus in its normal position. Cell viability was verified by dyeing of 1% neutral red solution. Above 90% isolated VHCs could maintained their shape and viability for 1h at the room temperature.Morphological criteria for VHC viablity were presented based on published cochlear outer hair cell morphological studies. This technique can contributed to investigations on the physiology of mammalian vestibular cells,and on the mechanisms of some diseases.

Key words: inner ear, vestibular hair cells, viable time, guinea pig

中图分类号:

R322.93

刘芝源;宋本波;王淑春. 豚鼠内耳前庭感觉毛细胞的分离[J]. 首都医科大学学报, 1994, 15(2): 112-114.

Liu Zhiyuan;Song Benbo;Wang Shuchun. Isolation and Identification of Inner Ear Vestibular Hair Cells of Guinea Pig[J]. Journal of Capital Medical University, 1994, 15(2): 112-114.

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