Abstract:

The aim was to investigate the effect of PD98059 on heart function endured 11, 12-EET a nd ischemia preconditioning and know about the effect of the expression of phosp horylated ERK1/ERK2 on the cardiac protection. Adopting ligating 60 min a nd releasing 30 min to make a ischemic/reperfusion model; ligating 5 min and rel ea sing 5 min (two times) to replicate ischemic preconditioning model. Observing th e changing of heart function during reperfusion process and detecting the expressi on of ERK1/ERK2 through western blot method. There was significant di fferen ce contrasting the heart function I/R group with SI+I/R and EET+I/R group, and t he heart function was significantly decreased after PD98059 infusion. The phosph orylated expression of ERK of I/R group was significantly lower than SI+I/R and E ET+I/R group, and the phosphorylated expression of ERK was significantly decreas ed after PD98059 infusion. It suggests that 6.24×10^-8 mol/L 11,12-EET h as the effect of protecting myocardium function that is inducted by increasing the expression of phosphorylated ERK1/ERK2.

Key words: 11, 12-epoxyeicosatrienoic acid, is chemia-reperfusion injury, ischemic preconditioning, excellular signal regulated kinase