人CD14基因克隆及基因结构分析

首都医科大学学报 ›› 2005, Vol. 26 ›› Issue (3): 296-298.

人CD14基因克隆及基因结构分析

侯本祥¹, 王慧², 张琛¹, 荫俊²

1. 首都医科大学口腔医学院;2. 军事医学科学院微生物流行病研究所

收稿日期:2005-01-10 修回日期:1900-01-01 出版日期:2005-06-24 发布日期:2005-06-24
通讯作者: 侯本祥

Gene Clone and Bio-information Analysis of Human CD14

Hou Benxiang¹, Wang Hui², Zhang Chen¹, Yin Jun²

1. Faculty of Stomatology, Capital University of Medical Sciences;2. Institute ofMicrobiology and Epidemiology, Academy of Military Medical Sciences

Received:2005-01-10 Revised:1900-01-01 Online:2005-06-24 Published:2005-06-24

摘要/Abstract

摘要：

目的扩增人CD14基因序列,对克隆基因进行结构及生物信息学分析.方法利用反转录PCR技术从U₉₃₇细胞总RNA中,扩增编码人CD14的基因序列,对基因结构及其编码的CD14成熟蛋白质进行了限制性酶切位点、蛋白质组成、相对分子质量及等电点、蛋白质稳定性、信号肽定位、蛋白质Prosite和2级结构等生物信息学分析.结果序列分析结果表明扩增的基因序列与文献报道完全一致,在此基础上获得了人CD14基因结构和生物信息学参数.结论成功获得人CD14基因综合参数,为进一步重组CD14表达、鉴定和结构功能研究提供了理论依据.

关键词: CD14, 基因克隆, 生物信息学

Abstract:

Objective To amplify the gene sequence of CD14, analyze the structure and bio-information of the cloned genes. Methods Human soluble CD14(sCD14) gene cDNA fragment was amplified from total RNA extracted from U₉₃₇ cells by RT-PCR. The bio-information of cloned CD14 gene and translated protein was then analyzed, DNA and amino sequence, protein prosite and stabilization, alpha-helix/Deleage and Roux were identified. Results The amplified nucleotide sequence fragment consistent completely with the published human CD14 sequence. Moreover, we knew clearly gene structure and bio-information parameter of human soluble CD14 according the sequence. Conclusion The co-index of the gene of CD14 is achieved successfully. These results make it possible to identify Human recombinant CD14 gene.

Key words: CD14, gene cloning, bio-information

中图分类号:

Q785

侯本祥;王慧;张琛;荫俊. 人CD14基因克隆及基因结构分析[J]. 首都医科大学学报, 2005, 26(3): 296-298.

Hou Benxiang;Wang Hui;Zhang Chen;Yin Jun. Gene Clone and Bio-information Analysis of Human CD14[J]. Journal of Capital Medical University, 2005, 26(3): 296-298.

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