大鼠原位肝移植后肿瘤坏死因子-α与供肝损伤的关系

首都医科大学学报 ›› 2005, Vol. 26 ›› Issue (4): 489-491.

大鼠原位肝移植后肿瘤坏死因子-α与供肝损伤的关系

夏仁品¹, 武聚山¹, 吴娟², 卢实春¹, 郭庆良¹, 黄建文³, 赵春惠¹, Terry B Strom⁴

1. 首都医科大学附属北京佑安医院肝胆外科;2. 仁寿县人民医院外科;3. 首都医科大学附属北京博爱医院泌尿外料;4. 美国哈佛大学医学院

收稿日期:2005-01-17 修回日期:1900-01-01 出版日期:2005-08-24 发布日期:2005-08-24

Relationship Between Tumor Necrosis Factor and Injuries of Liver Graft after Orthotopic Liver Transplantation in Rats

Xia Renpin¹, Wu Jushan¹, Wu Juan², Lu Shichun¹, Guo Qingliang¹, Huang Jianwen³, Zhao Chunhui¹, Terry B Strom⁴

1. Department of Hepatobiliary Surgery, Beijing Youan Hospital, Capital University of Medical Sciences;2. Department of Surgery, Renshou People Hospital;3. Department of Urology, Beijing Bo'ai Hospital, Capital University of Medical Sciences;4. Medical School, Harvard University

Received:2005-01-17 Revised:1900-01-01 Online:2005-08-24 Published:2005-08-24

摘要/Abstract

摘要：

目的观察肿瘤坏死因子-α(TNF-α)在肝移植后早期的活性变化及其与供肝损伤的关系。方法建立大鼠肝移植动物模型,观察其生存期外周血ALT变化、大鼠胆汁分泌量及移植肝门静脉血TNF-α活性。结果供肝冷冻保存4 h组及对照组移植大鼠存活期超过50 d,而冷冻保存8 h组大鼠无一长期存活(均＜3 d);对照组及4 h冷冻保存组胆汁分泌量显著多于8 h冷冻保存组(P＜0.05),8 h组ALT水平显著高于对照组及4 h组(P＜0.05);8 h组移植后TNF-α活性显著升高,且术后2 h达高峰,而4 h组仅在术后2 h轻度升高(P＜0.05)。结论肝移植早期TNF-α升高程度与供肝损伤程度有关.TNF-α可能源于移植肝的枯否细胞.

关键词: 肝移植, 肿瘤坏死因子-&alpha, 缺血再灌注损伤

Abstract:

Objective To study the relationship between tumor necrosis factor-α(TNF-α) levels and ischemia-reperfusion injury of liver grafts after different cold-storage time during the early stage after orthotopic liver transplantation in rats.Methods The survival periods and peripheral serum ALT levels were observed, and the secretion volume of bile as well as TNF-α activities of blood from portal vein of rats with liver grafts were examined.Results All the animals in groups of control and 4 h cold storage survived over 50 d, and group of 8 h cold storage survived less than 3 d. The secretion volume of bile were more in control and 4 h groups than that in 8 h group( P <0.05), and the ALT levels were lower in control and 4 h group than that in 8 h group( P <0.05). The activity of TNF-α was not detected in the control but elevated significantly in the 8 h group, especially 2 h after operation, and only elevated slightly in the 4 h group at the same time( P <0.05).Conclusion The upregulation of TNF-α is involved in the mechanism of ischemia-reperfusion injury of liver graft during the early stage after orthotopic liver transplantation. TNF-α may be originated from Kupffer cells of liver graft.

Key words: liver transplantation, tumor necrosis factor-&alpha, ischemia-reperfusion injury

中图分类号:

R657.3

夏仁品;武聚山;吴娟;卢实春;郭庆良;黄建文;赵春惠;Terry B Strom. 大鼠原位肝移植后肿瘤坏死因子-α与供肝损伤的关系[J]. 首都医科大学学报, 2005, 26(4): 489-491.

Xia Renpin;Wu Jushan;Wu Juan;Lu Shichun;Guo Qingliang;Huang Jianwen;Zhao Chunhui;Terry B Strom. Relationship Between Tumor Necrosis Factor and Injuries of Liver Graft after Orthotopic Liver Transplantation in Rats[J]. Journal of Capital Medical University, 2005, 26(4): 489-491.

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