地塞米松抑制培养牛眼小梁细胞AQP1表达

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (6): 833-835.

地塞米松抑制培养牛眼小梁细胞AQP1表达

赵丽¹, 康凤英², 王宁利³, 卢弘¹

1. 首都医科大学附属北京朝阳医院眼科;2. 山东潍坊医学院附属医院眼科;3. 首都医科大学附属北京同仁医院眼科中心

收稿日期:2006-02-17 修回日期:1900-01-01 出版日期:2006-12-24 发布日期:2006-12-24
通讯作者: 康凤英

Dexamethason Suppresses AQP1 Expression in Cultured Bovine Trabecular Meshwork Cells

Zhao Li¹, Kang Fengying², Wang Ningli³, Lu Hong¹

1. Department of Ophtalnology, Beijing Chaoyang Hospital, Capital University of Medical Sciences;2. Department of Ophthalmology, Affiliated Hospital of the Weifang Medical College;3. Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital University of Medical Sciences

Received:2006-02-17 Revised:1900-01-01 Online:2006-12-24 Published:2006-12-24

摘要/Abstract

摘要： 目的测定地塞米松对体外培养牛眼小梁细胞水通道蛋白-1 ( aquaporin1,AQP1 ) 表达的影响,探讨糖皮质激素性青光眼房水引流阻力的形成机制.方法将传3代的牛眼小梁细胞用数字表法随机分为对照和地塞米松处理组.将不同浓度的地塞米松(10^-5、10^-6、10^-7 mol/L)分别加入培养液持续培养14 d,免疫细胞化学方法检测小梁细胞AQP1表达水平,计算机图像分析软件测量细胞表面积.结果经不同浓度地塞米松处理后的小梁细胞AQP1表达量吸光度值A显著低于对照组(均P=0.000).10^-7 mol/L地塞米松作用下细胞表面积显著大于对照组,而10^-6 mol/L和10^-5 mol/L地塞米松作用下细胞表面积显著低于对照组(均P=0.000).结论地塞米松抑制牛眼小梁细胞AQP1表达,可能参与了糖皮质激素性青光眼的发生.

关键词: 小梁细胞, 水通道蛋白类, 地塞米松

Abstract: Objective To study the effects of dexamethason on AQP1 expression in bovine trabecular meshwork cells in vitro.Methods The third passage bovine trabecular meshwork cells were divided into control group and dexamethason-treated groups randomly.Different dosages of dexamethason(10(-5),10(-6),10(-7) mol/L) were added to cultures for 14 d.The AQP1 expressions were stained using immunocytochemistry method,and the cell areas were detected by the computer image analytical system.Results Comparisons were made between different groups.A means of AQP1 affected by dexamethason >10(-7) mol/L was much lower than that of control group > 10(-6) mol/L dexamethason > 10(-5) mol/L dexamethason.The mean cells areas were significantly increased in 10(-7) mol/L dexamethason treated group,but decreased in 10(-6) mol/L and 10(-5) mol/L dexamethason treated group compared with controls.Conclusion It is suggested that probably down-regulating the AQP1 express by dexamethason have potential significance in the development of steroid-induced glaucoma.

Key words: trabecular meshwork cells, aquaporins, dexamethason

中图分类号:

赵丽;康凤英;王宁利;卢弘. 地塞米松抑制培养牛眼小梁细胞AQP1表达[J]. 首都医科大学学报, 2006, 27(6): 833-835.

Zhao Li;Kang Fengying;Wang Ningli;Lu Hong. Dexamethason Suppresses AQP1 Expression in Cultured Bovine Trabecular Meshwork Cells[J]. Journal of Capital Medical University, 2006, 27(6): 833-835.

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