地塞米松体外对鼻息肉趋化因子生成的影响

doi:10.3969/j.issn.1006-7795.2024.02.010

首都医科大学学报

• 慢性鼻窦炎机制和临床研究 • 上一篇下一篇

地塞米松体外对鼻息肉趋化因子生成的影响

孟海洋^1,2,3, 王敏^1,2, 李颖^1,2, 王向东^1,2*#, 张罗^1,2*#

1.首都医科大学附属北京同仁医院耳鼻咽喉头颈外科、过敏科，北京 100730；2.北京市耳鼻咽喉科研究所,北京市中西医结合耳鼻咽喉科研究所，过敏性疾病北京实验室（北京市教育委员会），鼻病研究北京市重点实验室，耳鼻咽喉头颈科学教育部重点实验室（首都医科大学），北京 100005；3.北京市仁和医院耳鼻咽喉头颈外科，北京 102600

收稿日期:2023-01-18 出版日期:2024-04-25 发布日期:2024-04-25
通讯作者: 王向东, 张罗 E-mail:entwxd@vip.sina.com； dr.luozhang@139.com
基金资助:
国家重点研发计划项目（2022YFC2504100）,国家自然科学基金项目（81570893、81970852、82171110）,教育部长江学者创新团队项目（IRT13082）,中国医学科学院医学与健康科技创新工程项目资助（2019-I2M-5-022）,北京市自然科学基金项目（7162042、7222024）,首都临床特色诊疗技术研究及转化应用重点项目（Z211100002921057）,首都卫生发展科研专项重点攻关项目（2022-1-1091）,公益院所改革发展项目 (2019-10),北京市科技新星计划交叉合作项目(20220484226),北京市卫生系统高层次公共卫生人才建设项目领军人才项目（Lingjunrencai-01-08）。

Effect of dexamethasone on the chemokine production in nasal polyps in vitro

Meng Haiyang^1,2,3, Wang Min^1,2, Li Ying^1,2, Wang Xiangdong^1,2*#, Zhang Luo^1,2*#

1.Department of Otolaryngology Head and Neck Surgery and Department of Allergy, Beijing Tongren Hospital, Capital Medical University,Beijing 100730, China； 2.Beijing Institute of Otolaryngology, Beijing Institute of Integrated Traditional Chinese and Western Medicine in Otolaryngology, Beijing Laboratory of Allergic Diseases (Beijing Municipal Education Commission), Beijing Key Laboratory of Nasal Diseases, Key Laboratory of Otolaryngology Head and Neck Surgery (Capital Medical University)，Ministry of Education, Beijing 100005, China ； 3.Department of Otolaryngology Head and Neck Surgery, Beijing Renhe Hospital, Beijing 102600, China

Received:2023-01-18 Online:2024-04-25 Published:2024-04-25
Supported by:
This study was supported by National Key Research and Development Program (2022YFC2504100), National Natural Science Foundation of China(81570893,81970852,82171110). Changjiang Scholars Innovation Team of the Ministry of Education (IRT13082), Medical and Health Science and Technology Innovation Project of the Chinese Academy of Medical Sciences (2019-I2M-5-022), Natural Science Foundation of Beijing(7162042, 7222024), Capital Clinical Characteristic Diagnosis and Treatment Technology Research and Transformation Application Key Project (Z211100002921057), Capital Health Development Scientific Research Key Project (2022-1-1091), Reform and Development Project of Public Institutions (2019-10), New Star Cross Cooperation Project (20220484226), Leading Talents in Beijing Health System High-level Public Health Talent Construction Project (Lingjunrencai-01-08).

摘要/Abstract

摘要： 目的观察地塞米松（dexamethasone，DEX）对慢性鼻窦炎伴鼻息肉（chronic sinusitis with nasal polyps, CRSwNP）患者鼻息肉组织趋化因子生成的影响。方法收集CRSwNP患者鼻息肉组织，制备单细胞悬液，体外分别用10、50和100 μmol/L DEX处理，24 h后检测细胞活性和细胞培养上清中趋化因子的浓度。结果 10、50和100 μmol/L DEX处理组与未处理组相比，活细胞百分比无显著差别；CC趋化因子配体2（CC chemokine ligand,CCL2）、CCL11、CXC趋化因子配体2（CXC chemokine ligand，CXCL2）和CXCL9水平显著降低；CCL20水平显著增高；CCL3、CCL4 、CCL5、CCL17和CXCL5水平没有变化。结论 DEX抑制鼻息肉组织单细胞悬液中多种趋化因子的生成，提示其可能通过此作用抑制炎性细胞的浸润和炎症反应的发生。

关键词: 慢性鼻窦炎合并鼻息肉, 地塞米松, 趋化因子

Abstract: Objective To observe the effect of dexamethasone (DEX) on the production of chemokines in nasal polyp tissue of patients with chronic sinusitis with nasal polyps (CRSwNP). Methods Dispersed nasal polyp cells were prepared from nasal polyp tissues of CRSwNP patients and then the cells were treated with 10, 50 and 100 μmol/L DEX. The cell viability and levels of chemokines in cell culture supernatant were detected 24 h after DEX treatments. Results Compared to the untreated group, the percentage of viable cells in the 10, 50 and 100 μmol/L DEX-treated groups were unchanged. The levels of CCL2, CCL11, CXCL2 and CXCL9 were significantly lower, CCL20 significantly higher, CCL3,CCL4, CCL5, CCL17 and CXCL5 unchanged.Conclusion DEX reduced the production of multiple chemokines in dispersed nasal polyp cells of nasal polyp tissue, which may inhibit infiltration of inflammatory cells and occurrence of inflammatory response.

Key words: chronic sinusitis with nasal polyps, dexamethasone, chemokine

中图分类号:

R765

孟海洋, 王敏, 李颖, 王向东, 张罗. 地塞米松体外对鼻息肉趋化因子生成的影响[J]. 首都医科大学学报, doi: 10.3969/j.issn.1006-7795.2024.02.010.

Meng Haiyang, Wang Min, Li Ying, Wang Xiangdong, Zhang Luo. Effect of dexamethasone on the chemokine production in nasal polyps in vitro[J]. Journal of Capital Medical University, doi: 10.3969/j.issn.1006-7795.2024.02.010.

参考文献

［1］ Schleimer R P. Immunopathogenesis of chronic rhinosinusitis and nasal polyposis［J］. Annu Rev Pathol, 2017, 12: 331-357.
［2］ Wang X D, Sima Y T, Zhao Y, et al. Endotypes of chronic rhinosinusitis based on inflammatory and remodeling factors［J］. J Allergy Clin Immunol, 2023, 151(2): 458-468.
［3］ Hughes C E, Nibbs R J B. A guide to chemokines and their receptors［J］. FEBS J, 2018, 285(16): 2944-2971.
［4］李颖, 王向东, 王鸿, 等. CC类趋化因子在慢性鼻窦炎的表达［J］. 首都医科大学学报, 2013, 34(6): 795-798.
［5］ De Corso E, Baroni S, Battista M, et al. Nasal fluid release of eotaxin-3 and eotaxin-2 in persistent sinonasal eosinophilic inflammation［J］. Int Forum Allergy Rhinol, 2014, 4(8): 617-624.
［6］ Wang C, Lou H, Wang X, et al. Effect of budesonide transnasal nebulization in patients with eosinophilic chronic rhinosinusitis with nasal polyps［J］. J Allergy Clin Immunol, 2015, 135(4): 922-929.
［7］ Fokkens W J, Lund V J, Hopkins C, et al. European position paper on rhinosinusitis and nasal polyps 2020［J］. Rhinology, 2020, 58(Suppl 29): 1-464.
［8］ Wang M, Zhang N, Zheng M, et al. Cross-talk between TH2 and TH17 pathways in patients with chronic rhinosinusitis with nasal polyps［J］. J Allergy Clin Immunol, 2019, 144(5): 1254-1264.
［9］ Zeng M, Li Z Y, Ma J, et al. Clarithromycin and dexamethasone show similar anti-inflammatory effects on distinct phenotypic chronic rhinosinusitis: an explant model study［J］. BMC Immunol, 2015, 16: 37.
［10］ Locati M, Curtale G, Mantovani A. Diversity, mechanisms, and significance of macrophage plasticity［J］. Annu Rev Pathol, 2020, 15: 123-147.
［11］ Shapouri-Moghaddam A, Mohammadian S, Vazini H, et al. Macrophage plasticity, polarization, and function in health and disease［J］. J Cell Physiol, 2018, 233(9): 6425-6440.
［12］ Banks C A, Schlosser R J, Wang E W, et al. Macrophage infiltrate is elevated in CRSwNP sinonasal tissue regardless of atopic status［J］. Otolaryngol Head Neck Surg, 2014, 151(2): 215-220.
［13］ Krysko O, Holtappels G, Zhang N, et al. Alternatively activated macrophages and impaired phagocytosis of S. aureus in chronic rhinosinusitis［J］. Allergy, 2011, 66(3): 396-403.
［14］ Wang X, Zhang N, Bo M, et al. Diversity of TH cytokine profiles in patients with chronic rhinosinusitis: a multicenter study in Europe, Asia, and Oceania［J］. J Allergy Clin Immunol, 2016, 138(5): 1344-1353.
［15］ Gevaert P, Han J K, Smith S G, et al. The roles of eosinophils and interleukin-5 in the pathophysiology of chronic rhinosinusitis with nasal polyps［J］. Int Forum Allergy Rhinol, 2022, 12(11): 1413-1423.
［16］ Delemarre T, Holtappels G, De Ruyck N, et al. A substantial neutrophilic inflammation as regular part of severe type 2 chronic rhinosinusitis with nasal polyps［J］. J Allergy Clin Immunol, 2021, 147(1): 179-188.e2.
［17］ Wen W P, Liu W L, Zhang L, et al. Increased neutrophilia in nasal polyps reduces the response to oral corticosteroid therapy［J］. J Allergy Clin Immunol, 2012, 129(6): 1522-1528.e5.
［18］ Wang L, Yang M, Wang X, et al. Glucocorticoids promote CCL20 expression in keratinocytes［J］. Br J Dermatol, 2021, 185(6): 1200-1208.
［19］ Zijlstra G J, Fattahi F, Rozeveld D, et al. Glucocorticoids induce the production of the chemoattractant CCL20 in airway epithelium［J］. Eur Respir J, 2014, 44(2): 361-370.

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地塞米松体外对鼻息肉趋化因子生成的影响

Effect of dexamethasone on the chemokine production in nasal polyps in vitro

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