IL-23在慢性鼻窦炎中的表达和作用

doi:10.3969/j.issn.1006-7795.2020.02.006

首都医科大学学报 ›› 2020, Vol. 41 ›› Issue (2): 178-182.doi: 10.3969/j.issn.1006-7795.2020.02.006

• 慢性鼻窦炎伴鼻息肉的基础研究 • 上一篇下一篇

IL-23在慢性鼻窦炎中的表达和作用

王敏, 李颖, 王向东, 张罗

首都医科大学附属北京同仁医院耳鼻咽喉头颈外科北京市耳鼻咽喉科研究所耳鼻咽喉头颈科学教育部重点实验室鼻病研究北京市重点实验室, 北京 100005

收稿日期:2020-01-14 出版日期:2020-04-21 发布日期:2020-04-16
通讯作者: 张罗 E-mail:dr.luozhang@139.com
基金资助:
国家重点研发计划（2016YFC0905200），国家自然科学基金（81630023、81570893），教育部长江学者及创新团队发展计划（IRT13082），北京市自然科学基金（7162042）。

Expression and role of IL-23 in chronic rhinosinusitis

Wang Min, Li Ying, Wang Xiangdong, Zhang Luo

Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing Institute of Otorhinolaryngology, Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Key Laboratory of Nasal Disease, Beijing 100005, China

Received:2020-01-14 Online:2020-04-21 Published:2020-04-16
Supported by:
This study was supported by National Key R&D Program of China （2016YFC0905200）， National Natural Science Foundation of China （81630023， 81570893）， Program for Changjiang Scholars and Innovative Research Team （IRT13082）， Natural Science Foundation of Beijing （7162042）.

摘要/Abstract

摘要： 目的探讨IL-23因子在慢性鼻窦炎中的表达和对辅助性T细胞17（T helper cell 17，Th17）免疫反应的调节作用。方法募集15例受试者（正常对照组），14例慢性鼻窦炎不合并鼻息肉（chronic rhinosinusitis without nasal polyps，CRSsNP）患者（CRSsNP组）和37例慢性鼻窦炎合并鼻息肉（chronic rhinosinusitis with nasal polyps，CRSwNP）患者（CRSwNP组）。取正常对照组受试者的下鼻甲，CRSsNP组患者的筛窦黏膜和CRSwNP组患者的鼻息肉（nasal polyps，NP）组织制备组织匀浆，检测组织匀浆中白细胞介素-23（interleukin-23，IL-23）、髓过氧化物酶（myelo-peroxidase，MPO）和IL-17蛋白的浓度；计数组织中性粒细胞数目和外周血中性粒细胞数目和百分比。取NP组织消化成单个细胞悬液，IL-23体外刺激24 h，然后检测NP组织单个细胞悬液中Th17相关因子的浓度。结果与正常对照组相比，IL-23因子在CRSsNP和CRSwNP组患者中的表达均没有差别。但是，IL-17+CRSwNP亚组中IL-23的表达显著高于正常对照组和IL-17-CRSwNP亚组。鼻黏膜组织IL-23浓度与鼻黏膜组织MPO浓度正相关，但是与外周血和组织中的中性粒细胞数目不相关。IL-23体外刺激NP组织单个细胞悬液能够促进Th17相关因子IL-17和IL-1β的生成，但是对Th17相关因子IL-8和粒细胞集落刺激因子（granulocyte-colony stimulating factor，G-CSF）生成没有影响。结论 IL-23因子在IL-17+CRSwNP中通过增强Th17反应发挥作用。

关键词: 慢性鼻窦炎, 白细胞介素-23, 辅助性T 细胞17, 中性粒细胞

Abstract: Objective To investigate the expression of interleukin-23(IL-23) in chronic rhinosinusitis and the regulation in T helper cell 17(Th-17) response. Methods Fifteen control subjects, 14 patients with chronic rhinosinusitis without nasal polyps(CRSsNP) and 37 patients with chronic rhinosinusitis with nasal polyps (CRSwNP) were recruited. Tissue samples were obtained from the inferior turbinates of control subjects, the ethmoid mucosa of patients with CRSsNP, and the nasal polyps(NP) of patients with CRSwNP, respectively; and the tissue homogenates were prepared. The concentrations of IL-23, myelo-peroxidase(MPO) and IL-17 in tissue homogenates were measured. The number of neutrophils in nasal tissue and the count and percent of neutrophils in peripheral blood were counted. Dispersed nasal polyp cells were prepared from fresh NP tissue and stimulated with IL-23 for 24h in vitro and the concentrations of Th17-related cytokines in the culture supernatants were measured. Results Compared to normal control, no change of IL-23 concentrations in tissue homogenates from CRSsNP and CRSwNP were observed. However, IL-23 concentration in IL-17+CRSwNP was significantly higher than that in the control and IL-17-CRSwNP. IL-23 concentration in tissue homogenates was positively correlated with tissue MPO concentration, but had no correlation with peripheral or tissue neutrophil number. IL-23 simulation promoted the production of IL-17 and IL-1β in dispersed nasal polyp cells, but the production of IL-8 and granulocyte colony stimulating factor(G-CSF) did not change. Conclusion IL-23 may play a role in IL-17+CRSwNP via increasing Th17 response.

Key words: chronic rhinosinusitis, interleukin-23(IL-23), T helper cell 17(Th17), neutrophil

中图分类号:

R765

王敏, 李颖, 王向东, 张罗. IL-23在慢性鼻窦炎中的表达和作用[J]. 首都医科大学学报, 2020, 41(2): 178-182.

Wang Min, Li Ying, Wang Xiangdong, Zhang Luo. Expression and role of IL-23 in chronic rhinosinusitis[J]. Journal of Capital Medical University, 2020, 41(2): 178-182.

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IL-23在慢性鼻窦炎中的表达和作用

Expression and role of IL-23 in chronic rhinosinusitis

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